Structural variations of a new fertility restorer gene,Rf20,underlie the restoration of wild abortive-type cytoplasmic male sterility in rice  

作　　者：Shufeng Song Yixing Li Mudan Qiu Na Xu Bin Li Longhui Zhang Lei Li Weijun Chen Jinglei Li Tiankang Wang Yingxin Qiu Mengmeng Gong Dong Yu Hao Dong Siqi Xia Yi Pan Dingyang Yuan Li Li 

机构地区：[1]State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Hunan Academy of Agricultural Sciences,Changsha 410125,China [2]College of Agronomy,Hunan Agricultural University,Changsha 410128,China [3]College of Plant Protection,Hunan Agricultural University,Changsha 410128,China [4]College of Tropical Crops,Hainan University,Haikou 570228,China [5]Longping Branch,College of Biology,Hunan University,Changsha 410125,China

出　　处：《Molecular Plant》2024年第8期1272-1288,共17页分子植物（英文版）

基　　金：Biological Breeding-Major Projects(2023ZD04076);National Key Research and Development Project(2022YFD1200800);Hunan Natural Science Foundation(2023JJ30442);Special Funds for the Construction of Innovative Provinces in Hunan Province(2021NK1002).

摘　　要：The discovery of a wild abortive-type(WA)cytoplasmic male sterile(CMS)line and breeding its restorer line have led to the commercialization of three-line hybrid rice,contributing considerably to global food security.However,the molecular mechanisms underlyingfertility abortion and the restoration of CMs-WA lines remain largely elusive.In this study,we cloned a restorer gene,Rf2o,following a genome-wide association study analysis of the core parent lines of three-line hybrid rice.We found that Rf20 was present in all core parental lines,but different haplotypes and structural variants of its gene resulted in differences in Rf2o expression levels between sterileand restored lines.Rf20 could restorepollen fertility in the CMS-WA line and was found to be responsible for fertility restoration in some CMs lines under high temperatures.In addition,we found that Rf20 encodes a pentatricopeptide repeat protein that competes with WA352 for binding with COX11.This interaction enhances Cox11's function as a scavenger of reactive oxygen species,which in turn restores pollenfertility.Collectively,ourstudysuggestsanewactionmodeforpentatricopeptide repeatproteins inthe fertilityrestorationofCMs lines,providing an essential theoretical basisforbreedingrobustrestorer lines and for overcoming high temperature-induced fertility recovery of some CMS lines.

关 键 词：COX11 cytoplasmic male sterility rf20 WA352 wild abortion 

分 类 号：S511[农业科学—作物学]