Hordedane diterpenoid phytoalexins restrict Fusarium graminearum infection but enhance Bipolaris sorokiniana colonization of barley roots  

作　　者：Yaming Liu Dario Esposto Lisa K.Mahdi Andrea Porzel Pauline Stark Hidayat Hussain Anja Scherr-Henning Simon Isfort Ulschan.Bathe Ivan F.Acosta Alga Zuccaro Gerd U.Balcke Alain Tissier 

机构地区：[1]Department of Cell and Metabolic Biology,Leibniz Institute of Plant Biochemistry,Halle,Germany [2]Institute for Plant Sciences,Cluster of Excellence on Plant Sciences(CEPLAS),Cologne Biocenter,University of Cologne,Cologne,Germany [3]Department of Bioorganic Chemistry,Leibniz Institute of Plant Biochemistry,Halle,Germany [4]Max Planck Institute for Plant Breeding Research,Cologne,Germany [5]Horticultural Sciences,University of Florida,Gainesville,FL,USA.

出　　处：《Molecular Plant》2024年第8期1307-1327,共21页分子植物（英文版）

基　　金：TI 800/7-1 and TI 800/7-2(SPP 2125 DECRyPT)from the Deutsche Forschungsgemeinschaft,Germany,to A.T.We also acknowledge support from the Cluster of Excellence on Plant Sciences(CEPLAS);the Deutsche Forschungsgemeinschaft under Germany's ExcellenceStrategy-EXC 2048/1-Project ID:390686111and the grant ZU263/11-2(SPP 2125DECRyPT)to A.Z.

摘　　要：Plant immunity is a multilayered process that includes recognition of patterns or effectors from pathogens to elicit defense responses.These include the induction of a cocktail of defense metabolites that typically restrict pathogen virulence.Here,we investigate the interaction between barley roots and the fungal pathogens Bipolaris sorokiniana(Bs)and Fusarium graminearum(Fg)at the metabolite level.We identify hordedanes,a previously undescribed set of labdane-related diterpenoids with antimicrobial properties,as critical players in these interactions.Infection of barley roots by Bs and Fg elicits hordedane synthesis from a 60o-kb gene cluster.Heterologous reconstruction of the biosynthesis pathway in yeast and Nicotiana benthamiana produced several hordedanes,including one of the most functionally decorated products 19-β-hydroxy-hordetrienoic acid(19-OH-HTA).Barley mutants in the diterpene synthase genes of this cluster are unable to produce hordedanes but,unexpectedly,show reduced Bs colonization.By contrast,colonization by Fusarium graminearum,another fungal pathogen of barley and wheat,is 4-fold higher in the mutants completely lacking hordedanes.Accordingly,19-OH-HTA enhances both germination and growth of Bs,whereas it inhibits other pathogenic fungi,including Fg.Analysis of microscopy and transcriptomics data suggest that hordedanes delay the necrotrophic phase of Bs.Taken together,these results show that adapted pathogens such as Bs can subvert plant metabolic defenses to facilitate root colonization.

关 键 词：BARLEY Hordeum vulgare diterpenoid phytoalexins gene cluster pathogenic fungi Bipolaris sorokininiana Fusarium graminearum 

分 类 号：Q94[生物学—植物学]