2020-2022年某儿童专科医院铜绿假单胞菌分离株耐药性及Ⅰ类整合子变化趋势  

Changing trends of drug resistance and class Ⅰ integron of Pseudomonas aeruginosa isolates in a children's hospital from 2020 to 2022

作　　者：钱扬会 齐永志 李晓燕 刘晓菁 薛娟 QIAN Yang-hui;QI Yong-zhi;LI Xiao-yan;LIU Xiao-jing;XUE Juan(Beijing Jingdu Children's Hospital,Beijing 102208,China;不详)

机构地区：[1]北京京都儿童医院检验科,北京102208 [2]解放军总医院第六医学中心,北京100048

出　　处：《中华医院感染学杂志》2024年第15期2372-2376,共5页Chinese Journal of Nosocomiology

基　　金：解放军总医院第六医学中心创新培育基金(CXPY201902)。

摘　　要：目的检测临床分离铜绿假单胞菌耐药性及Ⅰ类整合子变化.方法北京京都儿童医院2020年1月—2022年12月临床分离的铜绿假单胞菌标本根据采集时间被分为三组:2020年、2021年和2022年.采用法国生物梅里埃公司的VITEK-2 Compact研究其对12种常用抗菌药物的耐药性,采用聚合酶链式反应(PCR)扩增检测Ⅰ类整合子.结果2020组、2021组和2022组分别检出铜绿假单胞菌为46、57和72株;铜绿假单胞菌对12种常用抗菌药物耐药性增长不明显,Ⅰ类整合子阳性菌检出率逐年增加,且Ⅰ类整合子阳性铜绿假单胞菌耐药性高于阴性菌(P<0.05).结论儿童专科医院铜绿假单胞菌耐药性较高,Ⅰ类整合子阳性铜绿假单胞菌检测比例增加明显.需要加强医院感染的检测和耐药菌监控,进一步规范抗菌药物使用.OBJECTIVE To investigate the drug resistance and classⅠintegron in clinical isolated Pseudomonas aeruginosa(PA).METHODS Clinically isolated PA specimens from Jingdu Children's Hospital of Beijing from Jan 2020 to Dec 2022 were divided into three groups according to the collection time(2020,2021 and 2022,re-spectively).VITEK-2 Compact(BioMérieux France)was used to study the drug resistance of 12 common antimi-crobials.ClassⅠintegrons were detected by polymerase chain reaction(PCR)assay.RESULTS A total of 46,57 and 72 strains of PA were detected in the 2020,2021 and 2022 groups,respectively.There was no significant in-crease in PA resistance to 12 common antibiotics.The detection rate of classⅠintegron positive bacteria increased year by year,and the drug resistance of classⅠintegron-positive PA was higher than that of negative bacteria(P<0.05).CONCLUSION The drug resistance of PA in children's hospital is high,with a significant increase in the proportion of classⅠintegron-positive PA.It is necessary to strengthen the detection of nosocomial infections and the monitoring of drug-resistant bacteria,and to further standardize the use of antibiotics.

关 键 词：儿童 铜绿假单胞菌 Ⅰ类整合子 耐药性 抗菌药物 医院感染 

分 类 号：R446.5[医药卫生—诊断学]