未足月胎膜早破并发绒毛膜羊膜炎患者胎盘TLR4及NF-κB和TRAF6蛋白表达水平及母婴结局  

Expressions of TLR4,NF-κB and TRAF6 proteins in placenta of preterm premature rupture of fetal membrane patients complicated with chorioamnionitis and matenal and infant outcomes

作　　者：何胜悦 刘宇[1] 梁艳 张英[1] 李国芸[1] HE Sheng-yue;LIU Yu;LIANG Yan;ZHANG Ying;LI Guo-yun(Xinriang Central Hospital,Xinriang,Henan 453000,China)

机构地区：[1]新乡市中心医院产一科,河南新乡453000

出　　处：《中华医院感染学杂志》2024年第15期2382-2386,共5页Chinese Journal of Nosocomiology

基　　金：河南省医学科技攻关计划(联合共建)项目(LHGJ20191322)。

摘　　要：目的探讨未足月胎膜早破患者胎盘Toll样受体4(TLR4)、核转录因子-κB(NF-κB)、肿瘤坏死因子受体相关因子6(TRAF6)蛋白表达与绒毛膜羊膜炎感染的发生、分期的关系及母婴结局。方法回顾性分析2020年6月-2023年6月新乡市中心医院收治的263例未足月胎膜早破患者的临床资料,根据是否为绒毛膜羊膜炎感染分为绒毛膜羊膜炎感染组/非绒毛膜羊膜炎感染组(98例/165例);采用免疫印迹试验检测胎盘TLR4、NF-κB、TRAF6蛋白表达水平;比较绒毛膜羊膜炎感染组和非绒毛膜羊膜炎感染组胎盘TLR4、NF-κB、TRAF6蛋白表达水平;比较绒毛膜羊膜炎早期组、中期组和晚期组胎盘TLR4、NF-κB、TRAF6蛋白表达水平;比较绒毛膜羊膜炎感染组和非绒毛膜羊膜炎感染组母婴结局;采用Spearman法分析未足月胎膜早破患者胎盘TLR4、NF-κB、TRAF6水平变化与不同分期绒毛膜羊膜炎感染的相关性。结果绒毛膜羊膜炎感染组胎盘TLR4、NF-κB、TRAF6蛋白水平均高于非绒毛膜羊膜炎感染组(P<0.05);中期组和晚期组胎盘TLR4、NF-κB、TRAF6蛋白水平较早期组高(P<0.05),晚期组胎盘TLR4、NF-κB、TRAF6蛋白水平较中期组高(P<0.05);绒毛膜羊膜炎感染组宫内窘迫、产后出血、羊水过少、呼吸窘迫综合征、新生儿窒息、死亡占比均高于非绒毛膜羊膜炎感染组(P<0.05);Spearman相关性分析结果显示,TLR4、NF-κB、TRAF6水平与绒毛膜羊膜炎分期呈正相关(P<0.05)。结论未足月胎膜早破患者绒毛膜羊膜炎感染后胎盘TLR4、NF-κB、TRAF6蛋白水平呈高表达,三者与绒毛膜羊膜炎分期密切相关,且未足月胎膜早破患者绒毛膜羊膜炎感染对母婴结局不利。OBJECTIVE To investigate the correlation of the protein expression of toll-like receptor 4(TLR4),nu-clear transcription factor-κB(NF-κB)and tumor necrosis factor receptor-related factor 6(TRAF6)in placenta with the occurrence,the stage of chorioamniotic infection and maternal and infant outcomes in patients with pre-term premature rupture of fetal membrane.METHODS The clinical data of 263 patients with preterm premature rupture of membranes admitted to Xinxiang Central Hospital from Jun 2020 to Jun 2023 were retrospectively ana-lyzed.According to whether the type of the infection was chorioamniotic infection,the patients were divided into the chorioamniotic infection group and non-chorioamniotic infection group(98 cases/165 cases).The expression levels of TLR4,NF-κB and TRAF6 in placenta were detected by western blot assay and compared between the chorioamniotic infection group and the non-chorioamniotic infection group.The expression levels of TLR4,NF-κB and TRAF6 in placenta of the early,the middle and the late chorioamniotic infection groups were compared.The outcomes of maternal and infant between the chorioamniotic infection group and the non-chorioamniotic infection group were compared.Spearman method was used to analyze the correlation between levels of TLR4,NF-κB,TRAF6 and chorioamniotic infection in patients with preterm premature rupture of membranes.RESULTS The protein levels of TLR4,NF-κB and TRAF6 in placenta of the chorioamniotic infection group were higher than those of the non-chorioamniotic infection group(P<0.05).The levels of TLR4,NF-κB and TRAF6 proteins in the middle-stage and late-stage groups were higher than those in the early-stage group(P<0.05),and the levels of TLR4,NF-κB and TRAF6 proteins in the late-stage group were higher than those in the middle-stage group(P<0.05).The rates of intrauterine distress,postpartum hemorrhage,oligohydramnios,respiratory distress syndrome,neonatal asphyxia and death in the chorioamniotic infection group were higher than those in the non-chorioamn

关 键 词：未足月胎膜早破 Toll样受体4 核转录因子-κB 肿瘤坏死因子受体相关因子6 绒毛膜羊膜炎 母婴结局 相关性 

分 类 号：R714.433[医药卫生—妇产科学]