当归芍药散对子宫内膜异位症模型大鼠上皮间充质转化相关因子的影响  

Effect of Danggui Shaoyao Decoction on the factors related to epithelial mesenchymal transformation in Endometriosis model rats

作　　者：钟莹 李钟彤 游琛 陈百晓 王颖杰 张亚萍 ZHONG Ying;LI Zhong-tong;YOU Chen;CHEN Bai-xiao;WANG Ying-jie;ZHANG Ya-ping(School of Basic Medical Science,Guangxi University of Chinese Medicine,Nanning 530200,China;Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530011,China)

机构地区：[1]广西中医药大学基础医学院,广西南宁530200 [2]广西中医药大学附属瑞康医院,广西南宁530011

出　　处：《时珍国医国药》2024年第7期1598-1601,共4页Lishizhen Medicine and Materia Medica Research

基　　金：广西自然科学基金(2020GXNSFAA297164);广西中医药大学博士科研启动基金(2017BS008);第五批全国中医临床优秀人才研修项目(国中医药人教函[2022]239号);国家教育部直属高校“西部之光”访问学者项目(教人司[2022]325号);广西中医药大学“桂派杏林青年英才”项目(2022C029);广西中医药大学“桂派中医药传承创新团队”项目(2022B002)。

摘　　要：目的探讨当归芍药散对子宫内膜异位症(EMs)模型大鼠异位内膜上皮间充质转化相关因子的影响。方法采用大鼠自体子宫内膜移植法构建EMs模型大鼠。将造模成功的48只EMs模型大鼠随机分为假手术组、模型组、当归芍药散低、中、高剂量组(3.96,7.92,15.84g·kg^(-1))、西药组(孕三烯酮胶囊0.25mg·kg^(-1)),每组8只。假手术组和模型组大鼠给予生理盐水(10mL·kg^(-1))灌胃。采用苏木素-伊红(HE)染色法对正常内膜及异位内膜进行组织形态学观察;免疫组化法检测正常子宫内膜与异位内膜组织的E-cadherin、N-cadherin蛋白表达;蛋白免疫印迹法(Western blot)检测正常内膜及异位组织β-catenin蛋白表达。结果与假手术组正常内膜相比,EMs各组异位子宫内膜组织的E-cadherin蛋白表达下降而β-catenin、N-cadherin蛋白含量升高(P<0.05)。与模型组相比,当归芍药散各剂量组的E-cadherin蛋白上调而β-catenin、N-cadherin蛋白表达含量下降(P<0.05)。结论当归芍药散对EMs模型大鼠的治疗作用可能与调节上皮间充质转化相关因子β-catenin以及E-cadherin、N-cadherin的表达有关。Objective To explore the effect of Danggui Shaoyao Decoction on the factors related to epithelial mesenchymal transformation of Endometriosis(EMs)model rats.Methods Construct an EMs rat model using autologous endometrial transplantation in rats.48 rats were randomly divided into a Sham surgery group,a model group,a low,medium and high dose group of Danggui Shaoyao Decoction(3.96,7.92,15.84g·kg^(-1)),and a western medicine group(0.25mg·kg^(-1)for Gestrinone capsules),with 8 rats in each group.Rats in the Sham surgery group and the model group were given normal saline(10mL·kg^(-1))by gavage.Using hematoxylin eosin(HE)staining method to observe the histological morphology of normal and ectopic endometrium;Immunohistochemical staining was used to detect the expression of E-cadherin and N-cadherin proteins in normal and ectopic endometrium tissues;Western blot detection of normal endometrium and ectopic tissuesβ-catenin protein expression.Results Compared with the normal endometrium in the Sham surgery group,the expression of E-cadherin protein in ectopic endometrium in the EMs group decreased the protein content ofβ-catenin and N-cadherin increased(P<0.05).Compared with the model group,the E-cadherin protein in each dose group of Danggui Shaoyao Decoction was upregulated the expression levels ofβ-catenin and N-cadherin proteins decreased(P<0.05).Conclusion The therapeutic effect of Danggui Shaoyao Decoction on EMs model rats may be related to regulating the expression of epithelial mesenchymal transition related factorsβ-catenin,E-cadherin and N-cadherin.

关 键 词：当归芍药散 子宫内膜异位症 上皮间充质转化 β联蛋白 钙黏蛋白 N钙黏蛋白 

分 类 号：R285.5[医药卫生—中药学]