日本血吸虫成虫和虫卵排泄分泌抗原对Ⅰ型糖尿病模型小鼠的影响  

Effect of AES and ESA of Schistosoma japonicum in the mice with typeⅠdiabetes

作　　者：王旗 章乐生[1] 汪峰峰[1] 汪敏 王毓洁[1] 马晓荷 李清越 操治国[1] WANG Qi;ZHANG Lesheng;WANG Fengfeng;WANG Min;WANG Yujie;MA Xiaohe;LI Qingyue;CAO Zhiguo(Anhui Provincial Institute of Schistosomiasis Control,Hefei 230031,Anhui Province,China)

机构地区：[1]安徽省血吸虫病防治研究所,安徽合肥230031

出　　处：《热带病与寄生虫学》2024年第4期239-243,共5页Journal of Tropical Diseases and Parasitology

基　　金：安徽省血吸虫病防治研究所青年科研基金项目(XF2021003);安徽省重点研究与开发计划项目(2022e07020003);安徽省学术和技术带头人及后备人选科研活动经费资助项目(2022H308)。

摘　　要：目的观察日本血吸虫成虫排泄分泌抗原(adult-worm excretory-secretory protein,AES)和虫卵排泄分泌抗原(excretory-secretory antigen of eggs,ESA)对Ⅰ型糖尿病小鼠的影响,并初步探讨其作用机制。方法将24只雄性昆明鼠随机分为空白对照组、PBS组、AES组和ESA组,每组6只。空白对照组不作任何处理,后3组给予链脲佐菌素(streptozotocin,STZ)溶液注射制备Ⅰ型糖尿病模型,建模成功后分别用PBS、AES、ESA腹部皮下多点免疫,1周免疫1次,持续免疫4周。免疫干预1周后开始检测小鼠血糖,持续检测4周;免疫干预4周后采用ELISA法测定小鼠血清中IL-4和IFN-γ水平变化,采用HE染色法检测小鼠胰腺组织病理变化。结果与PBS组相比,AES组和ESA组小鼠血糖水平从免疫后第2周起出现下降趋势,且ESA组小鼠血糖水平低于AES组,免疫后第2、3、4周小鼠血糖水平组间差异有统计学意义(F=1214.00、1055.00、683.64,P均<0.05)。各组小鼠血清IL-4和IFN-γ水平组间差异有统计学意义(F=146.84、21.11,P均<0.05),ESA组小鼠血清中IL-4水平[(61.45±6.14)pg/mL]高于PBS组[(21.96±3.98)pg/mL]和AES组[(49.31±3.19)pg/mL],IFN-γ水平[(129.48±36.75)pg/mL]低于PBS组[(316.43±66.38)pg/mL]和AES组[(212.09±70.89)pg/mL],差异均有统计学意义(P均<0.05)。PBS组小鼠的胰岛萎缩,数量减少,空泡样变性;ESA组小鼠有轻微胰岛萎缩;AES组介于PBS组和ESA组之间。结论日本血吸虫ESA对Ⅰ型糖尿病小鼠有一定保护作用,其机制可能是ESA刺激机体导致Th1反应抑制和Th2反应增强,表现为IL-4升高和IFN-γ下降,在一定程度上减轻小鼠胰腺组织的病理损伤。Objective To investigate the effects of adult-worm excretory-secretory protein(AES)and the excretory-secretory antigen of eggs(ESA)of Schistosoma japonicum on the mice with typeⅠdiabetes,and preliminarily explore the mechanisms of the antigens in the mice.Methods Twenty-four male KM mice were randomized to groups of blank control,PBS,AES and ESA(n=6 in each group).Mice in the blank control were free of any intervention,where-as those in PBS,AES and ESA groups were injected with streptozotocin(STZ)solution to prepare typeⅠdiabetes model.After successful modeling,the mice were immunized subcutaneously with PBS,AES or ESA,respectively,at multiple points in the abdomen,once a week,for 4 consecutive weeks.The blood glucose of mice was detected 1 week after immunization intervention and continued for 4 weeks.After 4 weeks of immune intervention,the serum levels of IL-4 and IFN-γwere measured by ELISA,and the pathological changes of pancreatic tissue were detected by HE staining.Results Compared with the PBS group,the blood glucose levels of mice in the AES and ESA groups showed a decreasing trend from the 2nd week after immunization,and the blood glucose levels of mice in the ESA group were lower than those in the AES group.The difference in blood glucose levels was significant between the groups at the 2nd,3rd and 4th weeks after immunization(F=1214.00,1055.00,683.64,all P<0.05),and the serum IL-4 and IFN-γlevels were significantly different between groups(F=146.84,21.11,both P<0.05).The serum IL-4 level in ESA group[(61.45±6.14)pg/mL]was higher than that in PBS group[(21.96±3.98)pg/mL]and AES group[(49.31±3.19)pg/mL],yet the IFN-γlevel[(129.48±36.75)pg/mL]was lower than that in PBS group[(316.43±66.38)pg/mL]and AES group[(212.09±70.89)pg/mL],with statistical difference(all P<0.05).Pancreatic islet atrophy,decrease in number and vacuole-like degeneration were observed in mice in PBS group,whereas pancreatic islet atrophy appeared slight in mice in ESA group,and the severity of pathological changes in the

关 键 词：日本血吸虫 成虫排泄分泌抗原 虫卵排泄分泌抗原 Ⅰ型糖尿病 

分 类 号：R383.24[医药卫生—医学寄生虫学] R587.1[医药卫生—基础医学]