高强度耐力运动对食源性肥胖小鼠肝脏CLK2、PGC-1α和PPARα表达的影响  

Effects of High-Intensity Endurance Exercise on the Expressions of CLK2,PGC-1αand PPARαin the Liver of Diet-Induced Obese Mice

作　　者：苏坤霞 张高飞 SU Kunxia;ZHANG Gaofei(Henan University of Chinese Medicine,Zhengzhou 451400,China;Zhoukou Normal University,Zhoukou 466001,China)

机构地区：[1]河南中医药大学,郑州451400 [2]周口师范学院,河南周口466001

出　　处：《河北体育学院学报》2024年第5期73-80,共8页Journal of Hebei Sport University

摘　　要：目的:研究高强度耐力运动对食源性肥胖小鼠肝脏蛋白CLK2、PGC-1α和PPARα的影响。方法:雄性4周龄C57BL/6J小鼠,先常规饲料适应性喂养1周,再通过8周高脂饲料喂养建立食源性肥胖小鼠模型,对照小鼠使用常规饲料喂养。肥胖小鼠经1周适应性跑台运动后进行高强度耐力跑台运动,对照小鼠不进行运动训练。运动训练进行8周后获取各组小鼠肝脏并称量肝脏质量;取部分肝脏组织进行HE染色,观察肝脏细胞形态,剩余肝脏组织通过Western Blot方法检测CLK2、PGC-1α和PPARα的表达情况并进行组间比较。结果:①食源性肥胖小鼠肝脏质量明显增加,肝细胞正常形态受到破坏;②高脂诱导能明显增加肝脏CLK2和PGC-1α表达,明显减少PPARα表达;③高强度耐力运动能有效减轻肥胖小鼠肝脏质量并恢复肝细胞正常形态;④高强度耐力运动能明显减少肥胖小鼠肝脏CLK2和PGC-1α表达,明显增加PPARα表达。结论:高强度耐力运动能够明显减少肝脏的脂质积累,改善肝脏脂质代谢情况。Objective:The purpose of this article is to study the effects of high-intensity endurance exercise on liver protein CLK2,PGC-1αand PPARαin food-induced obese mice.Methods:Male 4-week-old C57BL/6J mice were adaptively fed with conventional diet for 1 week,and then fed with high-fat diet for 8 weeks to establish a diet-induced obesity mice model.The control mice were fed with conventional diet.The obese mice were subjected to high-intensity endurance treadmill exercise after 1 week of adaptive treadmill exercise,while the control mice did not undergo exercise training.After 8 weeks of exercise training,the livers of mice in each group were obtained and weighed.Some liver tissues were taken for HE staining to observe the morphology of liver cells.The expression of CLK2,PGC-1αand PPARαin the remaining liver tissues was detected by Western Blot and compared between groups.Results:①The liver mass of food-induced obese mice was significantly increased,and the normal morphology of hepatocytes was destroyed.②High-fat diet could significantly increase the expression of CLK2 and PGC-1αin the liver,and significantly reduce the expression of PPARα.③High-intensity endurance exercise can effectively reduce the liver weight of obese mice and restore the normal morphology of liver cells.④High-intensity endurance exercise can significantly reduce the expression of CLK2 and PGC-1αin the liver of obese mice,and significantly increase the expression of PPARα.Conclusions:High-intensity endurance exercise can significantly reduce the accumulation of lipid and improve the lipid metabolism in the liver.

关 键 词：高强度 跑台 耐力运动 食源性肥胖小鼠 肝脏 CLK2 PGC-1Α PPARΑ 

分 类 号：G804.2[文化科学—运动人体科学]