基于酵母β-葡聚糖载体的口服OVA疫苗(WGP-OVA疫苗)诱导体液免疫及抗原特异性T细胞免疫反应  

Yeastβ-glucan vehicle based oral OVA vaccine(WGP-OVA vaccine)induces humoral and antigen-specific T cell immune responses

作　　者：白煜[1] 戚春建[1] 夏蕾[1] 何树燕 何流漾[1] BAI Yu;QI Chunjian;XIA Lei;HE Shuyan;HE Liuyang(Medical Research Center,the Affiliated Hospital of Nanjing Medical University,Changzhou No.2 People's Hospital,Changzhou 213000,China;Oncology Depart-ment,Jiangyin Hospital Affiliated to Nantong University,Jiangyin 214400,China)

机构地区：[1]南京医科大学附属常州市第二人民医院中心实验室,常州213000 [2]南通大学附属江阴医院肿瘤科,江阴214400

出　　处：《中国免疫学杂志》2024年第8期1579-1583,1589,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金(32270954);常州市科技计划项目(CJ20220082);江阴市卫健委面上项目(S202101)。

摘　　要：目的:探讨口服WGP-OVA疫苗对C57BL/6小鼠体液免疫及抗原特异性T细胞免疫的诱导作用。方法:分别连续给予C57BL/6小鼠口服PBS、WGP及WGP-OVA 17 d后,使用LEGENDplexTM多因子流式检测试剂盒检测给药后小鼠血清中IgG1、IgG2a、IgG3及IgM的含量;HE染色比较各组小鼠脾脏淋巴小结的大小。取小鼠腹股沟淋巴结(ILNs)制备单细胞悬液,流式细胞仪(FACS)检测淋巴结内F4/80+巨噬细胞及F4/80+SIINFEKL+巨噬细胞比例,分析WGP-OVA对巨噬细胞在ILNs内的浸润及抗原提呈情况;同时通过MHC Tetramer染色检测抗原特异性CD8+T细胞比例,明确WGP-OVA疫苗对T细胞免疫的诱导作用。结果:与PBS组相比,WGP-OVA能显著诱导IgG1、IgG2a、IgG3及IgM的分泌,增大脾脏内淋巴小结。此外,口服WGPOVA疫苗能促进F4/80+巨噬细胞向淋巴结浸润,诱导巨噬细胞对OVA的抗原提呈,同时增加淋巴结内OVA特异性CD8+CTLs的数量。结论:口服WGP-OVA疫苗能诱导C57BL/6小鼠体液免疫及抗原特异性T细胞免疫反应。Objective:To investigate humoral and antigen-specific T cell immune responses induced by oral administration of WGP-OVA in C57BL/6 mice.Methods:C57BL/6 mice were orally administered with PBS,WGP and WGP-OVA for 17 days,respectively.The serum immunoglobulin levels including IgG1,IgG2a,IgG3 and IgM of mice were detected using LEGENDplexTM Multi-Analyte Flow Assay Kit.HE staining was used to compare the size of lymphoid nodules in spleens.FACS was used to analyze the percentage of F4/80+macrophages and F4/80+SIINFEKL+macrophages in inguinal lymph nodes(ILNs).In addition,the T cell-mediated immunity stimulated by the WGP-OVA oral vaccine was analyzed by ILNs-infiltrating antigen-specific CD8+T cell using MHC Tetramer assay.Results:Oral administration of WGP-OVA significantly increased the contents of IgG1,IgG2a,IgG3 and IgM in the serum,and enlarged the lymphoid nodules in spleens,compared to those orally administered with PBS.Oral administration of WGP-OVA promoted the ILNs-infiltration of F4/80+macrophages,and enhanced the antigen-presentation of OVA in macrophages in ILNs.Moreover,the expansion of OVA specific CD8+CTLs in ILNs was also stimulated by WGP-OVA.Conclusion:WGP-OVA involved in both the humoral and antigen-specific T cell immune responses in C57BL/6 mice.

关 键 词：全β-葡聚糖颗粒 鸡卵清蛋白 口服疫苗 免疫球蛋白 抗原特异性T细胞 

分 类 号：R392[医药卫生—免疫学]