克氏原螯虾碳、氮稳定同位素周转与分馏特征研究  

Studies on the turnover and fractionation characteristics of stable isotopes of carbon and nitrogen in Procambarus clarkii

作　　者：陈晓龙 胡乐 熊飞[1,2] 喻记新 翟东东 刘红艳[1,2] 陈元元[1,2] CHEN Xiaolong;HU Le;XIONG Fei;YU Jixin;ZHAI Dongdong;LIU Hongyan;CHEN Yuanyuan(School of Life Sciences,Jianghan University,Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin,Wuhan 430056,China;Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances,Jianghan University,Wuhan 430056,China)

机构地区：[1]江汉大学生命科学学院,湖北省汉江流域特色生物资源保护开发与利用工程技术研究中心,武汉430056 [2]江汉大学持久性有毒污染物环境与健康危害湖北省重点实验室,武汉430056

出　　处：《淡水渔业》2024年第5期92-99,共8页Freshwater Fisheries

基　　金：国家自然科学基金(3210210459);江汉大学一流学科建设重大专项资助计划(2023XKZ025);湖北省高等学校优秀中青年科技创新团队计划项目(T2020034)。

摘　　要：为了解稳定同位素在克氏原螯虾(Procambarus clarkii)体内的周转与分馏特征,通过室内控制实验,研究了两种不同规格(虾种、成虾)克氏原螯虾在不同投饲条件下(饲料、鱼糜)的碳、氮稳定同位素周转半衰期和肌肉组织判别系数。结果表明,饲料组和鱼糜组虾种的生长系数分别为(0.0085±0.0007)d^(-1)、(0.0079±0.0012)d^(-1),成虾的生长系数分别为(0.0013±0.0001)d^(-1)、(0.0009±0.0004)d^(-1),不同投饲条件下克氏原螯虾生长系数差异不显著。虾种的相对增长率(1.60、1.57)大于成虾(1.08、1.05),不同投饲条件下克氏原螯虾相对增长率差异不大。相对于饲料组,鱼糜组虾种碳稳定同位素比值(δ^(13) C)在第7天出现显著差异,在28 d至56 d趋于稳定;成虾组δ^(13) C值在第14 d出现显著差异,在28 d至56 d趋于稳定;虾种和成虾氮稳定同位素比值(δ^(15) N)均在第14 d出现显著差异,在28 d至56 d趋于稳定。虾种碳稳定同位素周转速率(0.0208±0.0040)d^(-1)大于成虾(0.0184±0.0020)d^(-1)。虾种和成虾的碳同位素判别系数分别为0.89‰±0.15‰、1.06‰±0.16‰,氮同位素判别系数分别为3.42‰±0.08‰、3.63‰±0.13‰,虾种的碳同位素判别系数、氮同位素判别系数均小于成虾。研究结果可为应用碳、氮稳定同位素技术开展克氏原螯虾食性研究提供基础参数,提高食源分析的准确性。To understand the turnover and fractionation characteristics of carbon and nitrogen stable isotopes in Procambarus clarkii,the turnover half-life of carbon and nitrogen stable isotopes and the muscle tissue discrimination factor of two different sizes of P.clarkii(juvenile and adult)under different feeding conditions(feed and fish mince)were investigated in an indoor controlled experiment.The results showed that there were no significant differences in the growth coefficients between P.clarkii(juvenile)fed with feed(0.0085±0.0007)d^(-1) and those fed with fish mince(0.0079±0.0012)d^(-1).The differences in the growth coefficients between P.clarkii(adult)fed with feed(0.0013±0.0001)d^(-1) and those fed with fish mince(0.0009±0.0004)d^(-1) were no significant too.The relative growth rate of P.clarkii(juvenile)group(1.60,1.57)was higher than that of P.clarkii(adult)(1.08,1.05),while the relative growth rates between the feed and fish mince groups showed no significant differences.Compared to the feed group,the fish mince group exhibited a significant difference in the carbon stable isotope ratio(δ^(13) C)of the original crayfish species on the 7 d,which stabilized from 28 to 56 d.The carbon stable isotope ratio of P.clarkii(adult)group showed significant differences on the 14 d,also stabilizing from 28 to 56 d.Moreover,the nitrogen stable isotope ratios(δ^(15) N)of both P.clarkii(juvenile and adult)displayed significant differences on the 14th day,subsequently stabilizing from 28 to 56 d.The turnover rate of stable carbon isotopes in P.clarkii(juvenile)(0.0208±0.0040)d^(-1) was higher than that in P.clarkii(adult)(0.0184±0.0020)d^(-1).The carbon stable isotope discrimination factor of P.clarkii(juvenile and adult)were 0.89‰±0.15‰and 1.06‰±0.16‰,respectively.Similarly,the nitrogen stable isotope discrimination factor of P.clarkii(juvenile and adult)were 3.42‰±0.08‰and 3.63‰±0.13‰,respectively.It is worth noting that the carbon and nitrogen stable isotope discrimination factor of P.clarkii(j

关 键 词：克氏原螯虾(Procambarus clarkii) 碳周转速率 稳定同位素判别系数 碳稳定同位素比值 氮稳定同位素比值 

分 类 号：S966.12[农业科学—水产养殖]