同源细胞微移植技术联合A型肉毒毒素促进毛发再生的实验研究  

Experimental study of isogenous micro-grafting technique combined with botulinum toxin type A in promoting hair regeneration

作　　者：朱秋璇 宋采滢 程盛荣 陈文东 朱飞[1] Zhu Qiuxuan;Song Caiying;Cheng Shengrong;Chen Wendong;Zhu Fei(Dept of Plastic and Reconstructive Surgery,The First Affiliated Hospital of Anhui Medical University,Hefei 230022)

机构地区：[1]安徽医科大学第一附属医院整形外科,合肥230022

出　　处：《安徽医科大学学报》2024年第8期1417-1422,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽高校省级自然科学研究重点项目(编号:KJ2014A108)。

摘　　要：目的 探索同源细胞微移植(MG)技术及A型肉毒毒素(BTX-A)联合使用对小鼠毛发再生的影响。方法 选取45只C57BL/6小鼠,随机分为生理盐水组(NS组)、MG组(MB-0组)、MG+低剂量BTX-A组(MB-2组)、MG+中剂量BTX-A组(MB-10组)、MG+高剂量BTX-A组(MB-50组),共5组,用药后观察小鼠背部毛发生长情况,并在实验第7、14、21天采用苏木精-伊红(HE)染色评估毛囊生长情况、在第21天采用免疫组织化学染色评估血管内皮生长因子(VEGF)及β-catenin的表达情况。结果 经过MG及BTX-A治疗后,小鼠皮肤变黑时间缩短(P<0.05),第14天新生毛发覆盖率增高(P<0.05)。与NS组比较,其余各实验组毛囊数目增多(P<0.05),VEGF及β-catenin表达增多。结论 MG联合BTX-A对小鼠毛发再生有促进作用,其机制可能和促进血管生长及激活β-catenin信号有关。Objective To explore the effects of isogenous micro-grafting(MG)technology and the combined use of botulinum toxin type A(BTX-A)on hair regeneration in mice.Methods Forty-five C57BL/6 mice were randomly divided into five groups:normal saline group(NS group),MG group(MB-0 group),MG+low-dose BTX-A group(MB-2 group),MG+medium dose BTX-A group(MB-10 group),MG+high-dose BTX-A group(MB-50 group).The growth of hair on the backs of the mice was observed after the administration of the drugs,and the hair follicles were evaluated by hematoxylin-eosin(HE)staining on the 7th,14th,and 21st days of the experiment,and the expression of vascular endothelial growth factor(VEGF)andβ-catenin was evaluated by immunohistochemical staining on day 21.Results After treatment with micrograft and botulinum toxin type A,the skin darkening time was shortened(P<0.05),and the coverage rate of new hair increased on the 14 th day(P<0.05).Compared with the NS group,the number of hair follicles increased(P<0.05)and the expression of VEGF andβ-catenin increased in each other group.Conclusion Isogenous micro-grafting technology combined with botulinum toxin type A has a promotional effect on hair regeneration in mice,and the mechanism may be related to the promotion of vascular growth and activation ofβ-catenin signaling.

关 键 词：毛囊 毛发生长 A型肉毒毒素 微移植 

分 类 号：R62[医药卫生—整形外科]