毛茛中与苯丙烷生物合成途径相关的1R-MYB类转录因子的鉴定与分析  

Identification and analysis of 1R-MYB transcription factors regulating the phenylpropane biosynthesis pathway in Ranunculus japonicus Thunb.

作　　者：樊金世博 钟欣欣 单婷玉 张京晶 王郅炜 吴家文 Fan Jinshibo;Zhong Xinxin;Shan Tingyu;Zhang Jingjing;Wang Zhiwei;Wu Jiawen(Graduate School of Anhui University of Chinese Medicine,Hefei 230012,China;Key Laboratory of Xin’an Medicine,Ministry of Education,Anhui University of Chinese Medicine,Hefei 230038,China;Anhui Academy of Chinese Medicine,Hefei 230012,China;Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement,Hefei 230012,China)

机构地区：[1]安徽中医药大学研究生院,合肥230012 [2]安徽中医药大学科研实验中心,新安医学教育部重点实验室,合肥230038 [3]安徽省中医药科学院,合肥230012 [4]安徽道地中药材品质提升协同创新中心,合肥230012

出　　处：《植物科学学报》2024年第4期478-487,共10页Plant Science Journal

基　　金：国家重点研发计划项目(2017YFC1701600);安徽省自然科学基金项目(2008085MH268);安徽省高等学校科学研究项目(2022AH050474)。

摘　　要：1R-MYB家族在植物生长发育、抗胁迫、次生代谢物合成中具有重要的调控作用。本研究基于毛茛(Ranunculus japonicus Thunb.)的全长转录组测序数据库,分析了其1R-MYB家族成员的基序、系统进化关系、表达特性、空间结构及功能。结果共鉴定出221个1R-MYB基因,其中18个通过KEGG富集到苯丙烷生物合成途径,可能调控此途径中的过氧化物酶。研究结果为深入探究毛茛1R-MYB转录因子调控苯丙烷的生物合成机制奠定了基础。The 1R-MYB family plays a crucial role in plant growth,development,stress resistance,and secondary metabolite synthesis.A full-length transcriptome database for three different tissues of Ranunculus japonicus Thunb.was constructed using PacBio isoform sequencing and DNA nanoball sequencing.In this study,members of the 1R-MYB family were identified in the full-length transcriptome database of R.japonicus.Their motifs,family evolution,expression patterns,spatial structure,and functions were analyzed.In total,221 MYB-related genes were identified at the transcriptome level,with 181R-MYB transcription factors(TFs)found to be enriched in the phenylpropanoid biosynthesis pathway based on KEGG analysis,potentially regulating peroxidase activity.Overall,this study provides a foundation for further exploration of the regulatory functions of 1R-MYB TFs in secondary metabolite biosynthesis in R.japonicus.

关 键 词：毛茛 转录因子 全长转录组 1R-MYB 苯丙烷生物合成途径 

分 类 号：Q943.2[生物学—植物学]