Box-Behnken响应面法优化中药通便膏中番泻苷A提取工艺  

Optimization of Extraction Process of Sennoside A from Traditional Chinese Medicine Laxative Ointment by Box-Behnken Response Surface Method

作　　者：黄楠楠[1] 史宇宁 何育薇 贾力维[1] 王一格 田园[1] 吴丽红[1] HUANG Nannan;SHI Yuning;HE Yuwei;JIA Liwei;WANG Yige;TIAN Yuan;WU Lihong(Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《化学与粘合》2024年第5期432-435,486,共5页Chemistry and Adhesion

基　　金：黑龙江省教育科学规划重点课题(编号:GJB1422245);黑龙江省大学生创新创业训练计划项目(编号:202210228013);黑龙江中医药大学大学生创新创业训练计划项目(编号:X202110228016);黑龙江省中医药管理局项目(编号:ZHY2020-078);黑龙江中医药管理局中医药经典普及化研究专项课题(编号:ZYW2022-094)。

摘　　要：利用响应面法优化中药通便膏剂中番泻苷A的提取工艺。以大黄中番泻苷A的含量作为提取指标,采用紫外-可见分光光度法测定其吸光度,结合单因素实验数据,运用Design-Expert 13软件设计3因素3水平Box-Behnken实验,从而得出番泻苷A的最佳提取工艺。在水浴温度为79.4℃、料液比为10、乙醇浓度为81%的工艺条件下所得番泻苷A最大含量为1.999 4 mg/g。确定了以大承气汤为核心处方的通便膏剂中番泻苷A的最佳提取工艺。The response surface method optimizes the extraction process of sennoside A from traditional Chinese medicine laxative ointment.The content of sennoside A in rhubarb is selected as the extraction index,the absorbance is determined by ultraviolet-visible spectrophotometry;the three-factor three-level Box-Behnken experiment is designed by Design-Expert 13 software,combined with the single-factor experimental data,the optimal extraction process of sennoside A is obtained.When the water-bath temperature is 79.4 °C,the material-liquid ratio is 10 and the ethanol concentration is 81%,the maximum content of sennoside A obtained will be 1.999 4 mg/g.The experiment determines the optimal extraction process of sennoside A in laxative ointment whose core prescription is Dachengqi decoction.

关 键 词：响应面 番泻苷A 中药通便膏剂 提取工艺 

分 类 号：R284.2[医药卫生—中药学]