MVA titration by plaque assay using crystal violet staining in DF-1 cells  

作　　者：Santiago Navarro-Forero Lara Dsouza Zhilong Yang 

机构地区：[1]Department of Veterinary Pathobiology,School of Veterinary Medicine&Biomedical Sciences,Texas A&M University,College Station,TX,USA

出　　处：《One Health Advances》2023年第1期48-51,共4页全健康进展（英文）

摘　　要：Modified Vaccinia Ankara(MVA)is a highly promising vector for generating safe vaccine candidates against many pathogens,such as HIV-1,SARS-CoV-2,and influenza viruses.The gold standard method to titrate MVA involves visualizing MVA plaques in chicken embryo fibroblasts after immunostaining.However,this method is time-consuming and costly.In this study,we evaluated the visualization of MVA plaques formed in continuous chicken embryo fibroblasts DF-1 cells using crystal violet staining.We found that MVA titration by plaque assay using crystal violet staining in DF-1 cells yielded similar results to immunostaining,with substantially reduced time and costs.The MVA plaque assay by crystal violet staining in DF-1 cells is a reliable method with accurate results and low time and financial costs.

关 键 词：MVA Plaque assay Crystal violet staining DF-1 cell 

分 类 号：R186[医药卫生—流行病学]