Antimicrobial susceptibility testing in veterinary medicine:performance,interpretation of results,best practices and pitfalls  

作　　者：Andrea T.Feßler Yang Wang Claire R.Burbick Dubraska Diaz-Campos Virginia RFajt Sara D.Lawhon Xian-Zhi Li Brian V.Lubbers Kelli Maddock Ron A.Miller Mark G.Papich Shabbir Simjee Michael T.Sweeney Jeffrey L.Watts Congming Wu Jianzhong Shen Stefan Schwarz 

机构地区：[1]Institute of Microbiology and Epizootics,Centre for Infection Medicine,School of Veterinary Medicine,Freie Universität Berlin,Berlin,Germany [2]Veterinary Centre for Resistance Research(TZR),Freie Universität Berlin,Berlin,Germany [3]National Key Laboratory of Veterinary Public Health and Safety,College of Veterinary Medicine,China Agricultural University,Beijing,China [4]Key Laboratory of Animal Antimicrobial Resistance Surveillance,Ministry of Agriculture and Rural Affairs,College of Veterinary Medicine,China Agricultural University,Beijing,China [5]Department of Veterinary Microbiology and Pathology,College of Veterinary Medicine,Washington State University,Pullman,WA,USA [6]Department of Clinical Sciences,College of Veterinary Medicine,The Ohio State University,Columbus,OH,USA [7]Texas A&M University School of Veterinary Medicine,College Station,TX,USA [8]Veterinary Drugs Directorate,Health Canada,Ottawa,ON,Canada [9]Department of Clinical Sciences,College of Veterinary Medicine,Kansas State University,Manhattan,KS,USA [10]North Dakota State University Veterinary Diagnostic Laboratory,Fargo,ND,USA [11]U.S.Food and Drug Administration,Center for Veterinary Medicine,Rockville,MD,USA [12]College of Veterinary Medicine,North Carolina State University,Raleigh,NC,USA [13]Elanco Animal Health,Hook,UK [14]Zoetis,Inc.,Kalamazoo,MI,USA.

出　　处：《One Health Advances》2023年第1期62-77,共16页全健康进展（英文）

摘　　要：The performance of antimicrobial susceptibility testing(AST)of bacteria and the interpretation of AST results for bacteria isolated from animals are complex tasks which must be performed using standard published methodology and overseen by experts in clinical microbiology and in consultation with clinical pharmacologists.Otherwise,AST has significant potential for errors and mistakes.In this review,we provide guidance on how to correctly perform AST of bacteria isolated from animals and interpret the AST results.Particular emphasis is placed on the various approved or published methodologies for the different bacteria as well as the application of interpretive criteria,including clinical breakpoints and epidemiological cut-off values(ECVs/ECOFFs).Application of approved interpretive criteria and definitions of susceptible,susceptible dose-dependent,nonsusceptible,intermediate,and resistant for clinical breakpoints as well as wild-type and non-wildtype for ECVs,are explained and the difficulties resulting from the lack of approved clinical breakpoints for other bacteria,indications,and animal species is discussed.The requirement of quality controls in any AST approach is also emphasized.In addition,important parameters,often used in monitoring and surveillance studies,such as MIC50,MIC90,and testing range,are explained and criteria for the classification of bacteria as multidrug-resistant,extensively drug-resistant or pandrug-resistant are provided.Common mistakes are presented and the means to avoid them are described.To provide the most accurate AST,one must strictly adhere to approved standards or validated methodologies,like those of the Clinical and Laboratory Standards Institute or other internationally accepted AST documents and the detailed information provided therein.

关 键 词：Antimicrobial susceptibility testing Quality controls Clinical breakpoints Epidemiological cut-off values Multidrug resistance 

分 类 号：S859.7[农业科学—临床兽医学]