Bfra-loaded nanoparticles confer protection against paratuberculosis infection  

作　　者：Yiduo Liu Yulan Chen Zhengmin Liang Lijia Luo Yuanzhi Wang Haoran Wang Xin Ge Srinand Sreevatsan Xiangmei Zhou 

机构地区：[1]College of Veterinary Medicine,National Key Laboratory of Veterinary Public Health and Safety,China Agricultural University,Beijing,China [2]Pathobiology and Diagnostic Investigation College of Veterinary Medicine,Michigan State University,East Lansing,MI,USA

出　　处：《One Health Advances》2023年第1期162-174,共13页全健康进展（英文）

基　　金：supported by"National Key Research and Development Program(Project No.2021YFD1800405)";"National Natural Science Foundation of China(Project No.31873005,No.32172800)";"China Agriculture Research System(No.CARS-36)".

摘　　要：Paratuberculosis is a chronic wasting disease of granulomatous enteritis in ruminants caused by Mycobacterium avium subsp.paratuberculosis(M.paratuberculosis,MAP)resulting in heavy economic losses to dairy industries worldwide.Currently,commercial vaccines were not effective in preventing pathogen shedding and were reported with serious side effects.To develop a novel and smarter paratuberculosis vaccine,we utilized PLGA nanoparticles encapsulating the Bfra antigen(Bfra-PLGA NPs).We observed that mice vaccinated with Bfra-PLGA NPs exhibited an enhanced secretory IFN-γ,CD4+T cells response and antibody IgG against MAP infection.In addition,secretions of the inflammatory cytokine TNF-αand IL-10 were increased following treatment with Bfra-PLGA NPs.A significant reduction in bacterial load was observed in the livers and spleens of animals vaccinated with Bfra-PLGA NPs.Furthermore,Bfra-PLGA NPs were effective to alleviate the pathological lesions of livers in mice.Overall,our approach provides a rational basis for employing PLGA nanoparticles to develop improved vaccines that induced protective immunity against paratuberculosis.

关 键 词：Mycobacterium avium subsp.paratuberculosis PLGA nanoparticles Bfra Immune responses Novel vaccine 

分 类 号：TB383[一般工业技术—材料科学与工程]