A free elution immunoassay for chloramphenicol in chicken and egg after magnetic ZIF-8 adsorption separation  

作　　者：Liang Zhang Sihan Wang Jincheng Xiong Yuliang Xv Jie Xie Zhanhui Wang Congming Wu Haiyang Jiang Jianzhong Shen 

机构地区：[1]Department of Veterinary Pharmacology and Toxicology,National Key Laboratory of Veterinary Public Health Security,Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety,Beijing Laboratory for Food Quality and Safety,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China [2]Technology Innovation Center for Food Safety Surveillance and Detection,Sanya Institute of China Agricultural University,Hainan 572025,China [3]Technology Innovation Center of Mass Spectrometry for State Market Regulation,Center for Advanced Measurement Science,National Institute of Metrology,Beijing 100029,China

出　　处：《One Health Advances》2023年第1期213-223,共11页全健康进展（英文）

基　　金：supported by the National Key Research and Development Program of China(No.2020YFF01014605).

摘　　要：The excellent adsorption and rapid separation capabilities of magnetic MOFs make them desirable pretreatment materials for solid substrates.In this study,magnetic ZIF-8(mZIF-8)was synthesized in situ by a one-step method in the aqueous solution of magnetic beads.Isothermal adsorption verified that the maximum adsorption capacity of(011)crystal-exposed rhombic dodecahedral ZIF-8 for chloramphenicol was up to 128.31 mg/g(mZIF-8=67.18 mg/g).Kinetic adsorption revealed that the type of ZIF-8/mZIF-8 adsorption on chloramphenicol belongs to the pseudo-secondary adsorption kinetics of chemical monomolecular layers.Characterization by FTIR,XPS,and XRD revealed that mZIF-8 interacts with chloramphenicol mainly byπ-πelectron stacking,electrostatic attraction,and hydrogen bonding interaction.ELISA confirmed that chloramphenicol remained antigenically active after adsorption by mZIF-8.The adsorption and separation of chloramphenicol residues in chicken and egg were completed by mZIF-8 within 20 min.mZIF-8 can be used directly for elution-free ELISA after the adsorption of chloramphenicol.The limits of detection(IC10)of the mZIF-8+ELISA in chicken and eggs were 1.18 ng/mL and 0.64 ng/mL,respectively.mZIF-8 is expected to be used as a magnetic solid-phase extraction material for the rapid pretreatment of antibiotic residues in other complex solid matrices.

关 键 词：Magnetic metal-organic frameworks PRETREATMENT Elution-free CHLORAMPHENICOL Enzyme-linked immunosorbent assay 

分 类 号：TB39[一般工业技术—材料科学与工程]