ISO-FLUCS:symmetrization of optofluidic manipulations in quasi-isothermal micro-environments  

作　　者：Antonio Minopoli Susan Wagner Elena Erben Weida Liao Iliya D.Stoev Eric Lauga Moritz Kreysing 

机构地区：[1]Max-Planck-Institute of Molecular Cell Biology and Genetics,Dresden,Germany [2]Department of Applied Mathematics and Theoretical Physics,University of Cambridge,Cambridge,UK [3]Center for Systems Biology Dresden,Dresden,Germany [4]Institute of Biological and Chemical Systems-Biological Information Processing,Karlsruhe Institute of Technology,Karlsruhe,Germany

出　　处：《eLight》2023年第1期105-121,共17页e光学（英文）

基　　金：funding by the Max Planck Society,the Karlsruhe Institute of Technology;support by the European Research Council,in particular the ERC Starting Grant GHOSTs(Grant No.853619);support by the Volkswagen Foundation(Life!Grant No.92772);the Max Planck Society,Karlsruhe Institute of Technology;the Hector Foundation;funding by the Deutsche Forschungsgemeinschaft(DFG,German Research Foundation)under Germany’s Excellence Strategy-2082/1-390761711;funding from the Engineering and Physical Sciences Research Council(EPSRC studentship).

摘　　要：Recently,it has been demonstrated that thermoviscous flows can be used for a range of fine micromanipulations,such as moving the cytoplasm of cells and developing embryos,intracellular rheology,and femtonewton-range force measurements.These flows,also known as focused-light-induced cytoplasmic streaming(FLUCS),are induced by mid-infrared laser scanning of a temperature spot through the sample.However,localized laser scanning can inflict temperature perturbations of several Kelvins on the sample,potentially eliciting unspecific biological responses.In this study,we demonstrate how exploiting symmetry relations during laser scanning effectively disentangles laser heating and flow induction.We introduce flow-neutral scan sequences that use dynamic photothermal stimuli and spatiotemporal symmetry relations of scanning bridging up to three distinct time scales.We leverage further insights from a recently published analytical model of flow fields to present quasi-homogenous temperature distributions that leave flow lines and their local and directed character largely invariant.We present practical,intuitive solutions through predesigned sets of scan lines with near isothermal distributions and demonstrate that they are sufficient to generate and control flows in Caenorhabditis elegans embryos on a magnitude well in excess of endogenous flow velocities.Our results enable the separation of two previously tightly linked classes of physical stimuli,introduce a new,even less invasive standard for performing FLUCS perturbations,and pave the way for new unexplored avenues in the fields of soft matter and biomedicine.

关 键 词：QUASI environments SYMMETRY 

分 类 号：O43[机械工程—光学工程]