肠制大黄对便秘模型小鼠空腹血糖的影响  

Effect of Intestine-Processed Rhei Radix Et Rhizoma on Fasting Blood Glucose in Mice Model of Constipation

作　　者：黄林槿 饶啸天 郑培森 陈超 HUANG Linjin;RAO Xiaotian;ZHENG Peisen;CHEN Chao(Shantou Hospital of Traditional Chinese Medicine Affiliated to Guangzhou University of Chinese Medicine,ShantouGuangdong 515040,China)

机构地区：[1]广州中医药大学附属汕头中医院,广东汕头515040

出　　处：《新中医》2024年第17期196-199,共4页New Chinese Medicine

基　　金：2023年省科技创新战略专项市县科技创新支撑(大专项+任务清单)项目(STKJ2023005);汕头市科技计划医疗卫生类别项目(220510096490326)。

摘　　要：目的:观察猪大肠炮制大黄(简称肠制大黄)对便秘模型小鼠空腹血糖的影响,初步探究降糖的作用机制。方法:将50只昆明小鼠随机分成正常组10只和造模组40只,造模组小鼠连续4天给予洛哌丁胺混悬液8 mg/(kg·d)灌胃建立便秘小鼠模型。将32只成功造模的小鼠再随机分为模型组、肠制大黄组、乳果糖组、生大黄组各8只,在正常组中随机选取8只小鼠作为对照组。第5天开始,模型组、肠制大黄组、生大黄组、乳果糖组仍予洛哌丁胺混悬液8 mg/(kg·d)灌胃4天,每天灌胃2 h后肠制大黄组及生大黄组再分别给予肠制大黄混悬液及生大黄混悬液按0.6 g/(kg·d)灌胃,乳果糖组予乳果糖混悬液6 g/(kg·d)灌胃,正常组和模型组小鼠予蒸馏水按0.2 mL/10 g灌胃,各组均连续干预4 d。观察各组小鼠一般情况,最后一次灌胃后检测体质量;造模成功后及药物干预4 d后测得2次空腹血糖(FBG)。结果:与对照组比较,模型组小鼠体质量升高(P<0.05);与模型组比较,肠大黄组、乳果糖组、生大黄组小鼠体质量减轻(P<0.05);与肠大黄组比较,生大黄组小鼠体质量降低(P<0.05)。干预后,肠大黄组和生大黄组小鼠FBG较干预前降低(P<0.05),且肠大黄组和生大黄组小鼠FBG低于模型组(P<0.05)。肠大黄组药品不良反应发生率明显低于生大黄组,差异有统计学意义(P<0.05)。结论:猪大肠炮制大黄能够降低便秘模型小鼠的血糖,减轻生大黄峻下的作用,达到降糖兼改善便秘的功效,其机制可能与肠道菌群有关。Objective:To observe the effect of Rhei Radix et Rhizoma processed with pork intestines(referred to as intestine-processed Rhei Radix et Rhizoma)on the fasting blood glucose in mice model of constipation,and to preliminarily explore the mechanism of its hypoglycemic effect.Methods:A total of 50 Kunming mice were randomly divided into the normal group with 10 mice and the model establishment group with 40 mice.The model establishment group was given 8 mg/(kg·d)of Loperamide suspension by gavage for 4 consecutive days to establish the mice model of constipation.The 32 successfully modeled mice were then randomly divided into four groups:the model group,the intestine-processed Rhei Radix et Rhizoma group,the lactulose group,and the Rhei Radix et Rhizoma group,each consisting of 8 mice.Additionally,8 mice were randomly selected from the normal group to serve as the control group.Starting from the 5th day,the model group,the intestine-processed Rhei Radix et Rhizoma group,the lactulose group,and the Rhei Radix et Rhizoma group were still given 8 mg/(kg·d)of Loperamide suspension by gavage for 4 consecutive days.Two hours after each gavage,the intestine-processed Rhei Radix et Rhizoma group and the Rhei Radix et Rhizoma group were given their respective suspensions of intestine�processed Rhei Radix et Rhizoma or Rhei Radix et Rhizoma at a dosage of 0.6 g/(kg·d)for gastric gavage,while the lactulose group was given lactulose suspension at a dosage of 6 g/(kg·d)for gastric gavage.The normal group and the model group were given distilled water at a dosage of 0.2 mL/10 g for gastric gavage.All groups were continuously treated for 4 days.The general condition of the mice in each group was observed,and the body mass was measured after the last gavage;the fasting blood glucose(FBG)was measured twice after the model was successfully established and 4 days after drug intervention.Results:Compared with the control group,the body mass of the mice in the model group increased(P<0.05);compared with the model group,the body ma

关 键 词：空腹血糖 便秘 大黄 猪大肠 中药炮制 肠道菌群 

分 类 号：R285.5[医药卫生—中药学] R283[医药卫生—中医学]