基于遗传基因预测免疫细胞与骨髓炎的因果关系  

Causal relationship between immune cells and osteomyelitis through genetic prediction

作　　者：易颖 朱赟 朱寒霜 钱爱 王姜超 彭家龙 YI Ying;ZHU Yun;ZHU Hanshuang;QIAN Ai;WANG Jiangchao;PENG Jialong(Ear,Nose and Throat Department,the Second People’s Hospital of China Three Gorges University,the Second People’s Hospital of Yichang,Yichang 443000,Hubei,China;Graduate College,Hunan University of Chinese Medicine,Changsha 410208,Hunan,China;Rehabilitation Medicine Department,the Second People’s Hospital of China Three Gorges University,the Second People’s Hospital of Yichang,Yichang 443000,Hubei,China)

机构地区：[1]三峡大学附属第二人民医院宜昌市第二人民医院五官科,湖北宜昌443000 [2]湖南中医药大学研究生院,湖南长沙410208 [3]三峡大学附属第二人民医院宜昌市第二人民医院康复医学科,湖北宜昌443000

出　　处：《中国现代医生》2024年第24期77-81,共5页China Modern Doctor

摘　　要：目的利用孟德尔随机化方法预测免疫细胞与骨髓炎(osteomyelitis,OM)的因果关系。方法从英国生物样本库、大规模全基因组关联分析获取731个免疫细胞与OM的数据集。采用逆方差加权法、加权中位数法和MR-Egger法分别依次验证731个免疫细胞与OM的因果关系,并运用Cochra’s Q检验、MR-Egger截距测试、孟德尔随机多态性残差和离群值、留一法评价结果的稳定性和可靠性。结果共21个免疫细胞与OM存在因果关系(P<0.05),其中T调节细胞、经典树突状细胞、B细胞与OM因果关系更稳定(P<0.01),且T调节细胞(OR=0.925,β=–0.078)是降低OM风险的保护因素。经典树突状细胞(OR=1.084,β=0.081)及B细胞(OR=1.063,β=0.061)是OM风险增加的危险因素。结论21个免疫细胞在遗传上与OM风险具有相关性。Objective To predict the causal relationship between immune cells and osteomyelitis(OM)by using Mendelian randomization.Methods Data sets of 731 immune cells and osteomyelitis were obtained from the UK Biobank and the genome wide association study.The inverse variance weighted method,weighted median method,and MR-Egger method were employed sequentially to verify the causal relationship between the 731 immune cells and osteomyelitis.Cochran’s Q test,MR-Egger intercept test,mendelian randomization pleiotropy residual sum and outlier(MR-PRESSO),and leave-one-out analysis were evaluatede the stability and reliability of the results.Results A total of 21 immune cells were found to have a causal relationship with osteomyelitis(P<0.05).Among them,T regulatory cells,classical dendritic cells and B cells were exhibited more stable causal relationships with osteomyelitis(P<0.01).Specifically,T regulatory cells(OR=0.925,β=–0.078)were identified as protective factors reducing the risk of osteomyelitis,while classical dendritic cells(OR=1.084,β=0.081)and B cells(OR=1.063,β=0.061)were identified as risk factors increasing the risk of osteomyelitis.Conclusion Twenty-one immune cells are genetically associated with the risk of osteomyelitis.

关 键 词：免疫细胞 骨髓炎 孟德尔随机化 T调节细胞 经典树突状细胞 B细胞 

分 类 号：R593.22[医药卫生—内科学]