Skin 11β-hydroxysteroid dehydrogenase type 1 enzyme expression regulates burn wound healing and can be targeted to modify scar characteristics  

作　　者：Kevin H.-Y.Tsai Huaikai Shi Roxanne J.Parungao Sina Naficy Xiaotong Ding Xiaofeng Ding Jonathan J.Hew Xiaosuo Wang Wojciech Chrzanowski Gareth G.Lavery Zhe Li Andrea C.Issler-Fisher Jun Chen Qian Tan Peter K.Maitz Mark S.Cooper Yiwei Wang 

机构地区：[1]Adrenal Steroid Group,ANZAC Research Institute,Concord Hospital,The University of Sydney,Sydney,NSW 2137,Australia [2]Burns and Reconstructive Surgery Research Group,ANZAC Research Institute,Concord Hospital,The Uni-versity of Sydney,Sydney,NSW 2137,Australia [3]School of Chemical and Biomolecular Engineering,The University of Sydney,Sydney,NSW 2006,Australia [4]Jiangsu Provincial Engineering Research Centre of TCM External Medication Development and Application,Nanjing University of Chinese Medicine,Nanjing,Jiangsu,210023,China [5]Department of Burns and Plastic Surgery,Nanjing Drum Tower Hospital Clinical College,Nanjing University of Chinese Medicine,Nanjing,Jiangsu,210008,China [6]Heart Research Institute,The University of Sydney,Sydney,NSW 2006,Australia [7]Sydney Nano Institute,Sydney Pharmacy School,Faculty of Medicine and Health,The University of Sydney,Sydney,NSW2006,Australia [8]Department of Biosciences,Centre for Healthy Ageing and Understanding Disease,Nottingham Trent University,NG14BU,UK [9]Burns and Reconstructive Surgery Unit,Concord Repatriation General Hospital,Sydney,NSW 2137,Australia

出　　处：《Burns & Trauma》2023年第1期32-45,共14页烧伤与创伤（英文）

基　　金：supported by National Health and Medical Research Council(NHMRC)fund(APP1101879);National Science Foundation of China(82172217)and ANZAC Research Institute near miss funding.

摘　　要：Background:Excessive scarring and fibrosis are the most severe and common complications of burn injury.Prolonged exposure to high levels of glucocorticoids detrimentally impacts on skin,leading to skin thinning and impaired wound healing.Skin can generate active glucocorticoids locally through expression and activity of the 11β-hydroxysteroid dehydrogenase type 1 enzyme(11β-HSD1).We hypothesised that burn injury would induce 11β-HSD1 expression and local glucocorticoid metabolism,which would have important impacts on wound healing,fibrosis and scarring.We additionally proposed that pharmacological manipulation of this system could improve aspects of post-burn scarring.Methods:Skin 11β-HSD1 expression in burns patients and mice was examined.The impacts of 11β-HSD1 mediating glucocorticoid metabolism on burn wound healing,scar formation and scar elas-ticity and quality were additionally examined using a murine 11β-HSD1 genetic knockout model.Slow-release scaffolds containing therapeutic agents,including active and inactive glucocorticoids,were developed and pre-clinically tested in mice with burn injury.Results:We demonstrate that 11β-HSD1 expression levels increased substantially in both human and mouse skin after burn injury.11β-HSD1 knockout mice experienced faster wound healing than wild type mice but the healed wounds manifested significantly more collagen deposition,tensile strength and stiffness,features characteristic of excessive scarring.Application of slow-release prednisone,an inactive glucocorticoid,slowed the initial rate of wound closure but significantly reduced post-burn scarring via reductions in inflammation,myofibroblast generation,collagen production and scar stiffness.Conclusions:Skin 11β-HSD1 expression is a key regulator of wound healing and scarring after burn injury.Application of an inactive glucocorticoid capable of activation by local 11β-HSD1 in skin slows the initial rate of wound closure but significantlyimproves scar characteristics post burn injury.

关 键 词：Wound healing Burn injury SCARRING Skin 11β-HSD1 11β-HSD1 knockout Glucocorticoid metabolism Polycaprolactone scaffold Drug delivery 

分 类 号：R619.2[医药卫生—外科学]