A volar skin excisional wound model for in situ evaluation of multiple-appendage regeneration and innervation  

作　　者：Huanhuan Gao Yiqiong Liu Ziwei Shi Hongliang Zhang Mengyang Wang Huating Chen Yan Li Shaifei Ji Jiangbing Xiang Wei Pi Laixian Zhou Yiyue Hong Lu Wu Aizhen Cai Xiaobing Fu Xiaoyan Sun 

机构地区：[1]Research Center for Tissue Repair and Regeneration Affiliated to Medical Innovation Research Department and 4^(th)Medical Center,PLA General Hospital and PLA Medical College,PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury,Repair and Regeneration,Research Unit of Trauma Care,Tissue Repair and Regeneration,Chinese Academy of Medical Sciences,2019RU051,Beijing 100048,P.R.China [2]Institute of Chemistry,Chinese Academy of Sciences,2 Beiyi Street,Zhong guan cun,Beijing 100190,P.R.China [3]Research Institute of General Surgery,Department of General Surgery,the First Medical Center,PLA General Hospital,28 Fu Xing Road,Beijing 100853,P.R.China

出　　处：《Burns & Trauma》2023年第1期560-572,共13页烧伤与创伤（英文）

基　　金：supported in part by the National Nature Science Foundation of China[92268206,81830064];the CAMS Innovation Fund for Medical Sciences[CIFMS,2019-I2M-5-059];the Military Medical Research Projects[145AKJ260015000X,2022-JCJQ-ZB-09600,2020-JCJQZD-256-021];the Military Medical Research and Development Projects[AWS17J005,2019-126];the Specific Research Fund of The Innovation Platform for Academicians of Hainan Province[YSPTZX202317].

摘　　要：Background:Promoting rapid wound healing with functional recovery of all skin appendages is the main goal of regenerative medicine.So far current methodologies,including the commonly used back excisional wound model(BEWM)and paw skin scald wound model,are focused on assessing the regeneration of either hair follicles(HFs)or sweat glands(SwGs).How to achieve de novo appendage regeneration by synchronized evaluation of HFs,SwGs and sebaceous glands(SeGs)is still challenging.Here,we developed a volar skin excisional wound model(VEWM)that is suitable for examining cutaneous wound healing with multiple-appendage restoration,as well as innervation,providing a new research paradigm for the perfect regeneration of skin wounds.Methods:Macroscopic observation,iodine-starch test,morphological staining and qRT-PCR anal-ysis were used to detect the existence of HFs,SwGs,SeGs and distribution of nerve fibres in the volar skin.Wound healing process monitoring,HE/Masson staining,fractal analysis and behavioral response assessment were performed to verify that VEWM could mimic the pathological process and outcomes of human scar formation and sensory function impairment.Results:HFs are limited to the inter-footpads.SwGs are densely distributed in the footpads,scattered in the IFPs.The volar skin is richly innervated.The wound area of the VEWM at 1,3,7 and 10 days after the operation is respectively 89.17%±2.52%,71.72%±3.79%,55.09%±4.94%and 35.74%±4.05%,and the final scar area accounts for 47.80%±6.22%of the initial wound.While the wound area of BEWM at 1,3,7 and 10 days after the operation are respectively 61.94%±5.34%,51.26%±4.89%,12.63%±2.86%and 6.14%±2.84%,and the final scar area accounts for 4.33%±2.67%of the initial wound.Fractal analysis of the post-traumatic repair site for VEWM vs human was performed:lacunarity values,0.040±0.012 vs 0.038±0.014;fractal dimen-sion values,1.870±0.237 vs 1.903±0.163.Sensory nerve function of normal skin vs post-traumatic repair site was assessed:mechanical threshold,1.05±0.52 v

关 键 词：Wound healing Animal model Multiple appendages INNERVATION Regeneration evaluation Hair follicles Sweat gland Sebaceous gland 

分 类 号：R318[医药卫生—生物医学工程]