Engineering Nitrogenases for Synthetic Nitrogen Fixation:From Pathway Engineering to Directed Evolution  

作　　者：Emily M.Bennett James W.Murray Mark Isalan 

机构地区：[1]Department of Life Sciences,Imperial College London,London SW72AZ,UK.

出　　处：《BioDesign Research》2023年第1期25-36,共12页生物设计研究（英文）

基　　金：E.B.is supported by a BBSRC Doctoral Training Partnership Studentship(Project Reference:1949015).

摘　　要：Globally,agriculture depends on industrial nitrogen fertilizer to improve crop growth.Fertilizer production consumes fossil fuels and contributes to environmental nitrogen pollution.A potential solution would be to harness nitrogenases—enzymes capable of converting atmospheric nitrogen N2 to NH3 in ambient conditions.It is therefore a major goal of synthetic biology to engineer functional nitrogenases into crop plants,or bacteria that form symbiotic relationships with crops,to support growth and reduce dependence on industrially produced fertilizer.This review paper highlights recent work toward understanding the functional requirements for nitrogenase expression and manipulating nitrogenase gene expression in heterologous hosts to improve activity and oxygen tolerance and potentially to engineer synthetic symbiotic relationships with plants.

关 键 词：CROPS SYNTHETIC EVOLUTION 

分 类 号：Q34[生物学—遗传学]