Plant Promoters and Terminators for High-Precision Bioengineering  

作　　者：Emily G.Brooks Estefania Elorriaga Yang Liu James R.Duduit Guoliang Yuan Chung-Jui Tsai Gerald A.Tuskan Thomas G.Ranney Xiaohan Yang Wusheng Liu 

机构地区：[1]Department of Horticultural Science,North Carolina State University,Raleigh,NC 27607,USA. [2]Biosciences Division,Oak Ridge National Laboratory,Oak Ridge,TN 37831,USA. [3]The Center for Bioenergy Innovation,Oak Ridge National Laboratory,Oak Ridge,TN 37831,USA. [4]Warnell School of Forestry and Natural Resource,University of Georgia,Athens,GA 30602,USA. [5]Department of Plant Biology,University of Georgia,Athens,GA 30602,USA. [6]Department of Genetics,University of Georgia,Athens,GA 30602,USA. [7]Mountain Crop Improvement Lab,Department of Horticultural Science,Mountain Horticultural Crops Research and Extension Center,North Carolina State University,Mills River,NC 28759,USA.

出　　处：《BioDesign Research》2023年第1期86-105,共20页生物设计研究（英文）

基　　金：the USDA Floriculture and Nursery Research Initiative(FNRI)grant 8020-21000-071-23S;the USDA National Institute of Food and Agriculture(NIFA)Hatch project 02913;the Center for Bioenergy Innovation(CBI),which is a U.S.Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science.Oak Ridge National Laboratory is managed by UT-Battelle,LLC for the U.S.DOE under contract number DE-AC05-00OR22725。

摘　　要：High-precision bioengineering and synthetic biology require fine-tuning gene expression at both transcriptional and posttranscriptional levels.Gene transcription is tightly regulated by promoters and terminators.Promoters determine the timing,tissues and cells,and levels of the expression of genes.Terminators mediate transcription termination of genes and affect mRNA levels posttranscriptionally,e.g.,the 3′-end processing,stability,translation efficiency,and nuclear to cytoplasmic export of mRNAs.The promoter and terminator combination affects gene expression.In the present article,we review the function and features of plant core promoters,proximal and distal promoters,and terminators,and their effects on and benchmarking strategies for regulating gene expression.

关 键 词：expression PROMOTER PROXIMAL 

分 类 号：Q94[生物学—植物学]