Revealing the Host-Dependent Nature of an Engineered Genetic Inverter in Concordance with Physiology  

作　　者：Dennis Tin Chat Chan Geoff S.Baldwin Hans C.Bernstein 

机构地区：[1]Faculty of Biosciences,Fisheries and Economics,UiT,The Arctic University of Norway,9019 Tromsø,Norway. [2]Department of Life Sciences,Imperial College London,South Kensington,London SW72AZ,UK. [3]Imperial College Centre for Synthetic Biology,Imperial College London,South Kensington,London SW72AZ,UK. [4]The Arctic Centre for Sustainable Energy,UiT,The Arctic University of Norway,9019 Tromsø,Norway.

出　　处：《BioDesign Research》2023年第1期106-120,共15页生物设计研究（英文）

摘　　要：Broad-host-range synthetic biology is an emerging frontier that aims to expand our current engineerable domain of microbial hosts for biodesign applications.As more novel species are brought to“model status,”synthetic biologists are discovering that identically engineered genetic circuits can exhibit different performances depending on the organism it operates within,an observation referred to as the“chassis effect.”It remains a major challenge to uncover which genome-encoded and biological determinants will underpin chassis effects that govern the performance of engineered genetic devices.In this study,we compared model and novel bacterial hosts to ask whether phylogenomic relatedness or similarity in host physiology is a better predictor of genetic circuit performance.This was accomplished using a comparative framework based on multivariate statistical approaches to systematically demonstrate the chassis effect and characterize the performance dynamics of a genetic inverter circuit operating within 6 Gammaproteobacteria.Our results solidify the notion that genetic devices are strongly impacted by the host context.Furthermore,we formally determined that hosts exhibiting more similar metrics of growth and molecular physiology also exhibit more similar performance of the genetic inverter,indicating that specific bacterial physiology underpins measurable chassis effects.The result of this study contributes to the field of broad-host-range synthetic biology by lending increased predictive power to the implementation of genetic devices in less-established microbial hosts.

关 键 词：INVERTER Engine FORMALLY 

分 类 号：Q78[生物学—分子生物学]