Discordance between chromatin accessibility and transcriptional activity during the human primed-to-naïve pluripotency transition process  

作　　者：Zhifen Tu Yan Bi Tengyan Mao Hong Wang Shaorong Gao Yixuan Wang 

机构地区：[1]Translational Medical Center for Stem Cell Therapy&Institute for Regenerative Medicine,Shanghai East Hospital,School of Life Sciences and Technology,Tongji University,Shanghai 200120,China [2]Frontier Science Center for Stem Cell Research,Tongji University,1239 Siping Road,Shanghai 200092,China [3]Shanghai Key Laboratory of Maternal Fetal Medicine,Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital,School of Life Sciences and Technology,Tongji University,Shanghai 200092,China

出　　处：《Cell Regeneration》2023年第1期39-51,共13页细胞再生（英文）

基　　金：supported by the Ministry of Science and Technology of China(2021YFA11020000,2022YFC2702200 and 2018YFA0108900);the National Natural Science Foundation of China(NSFC)(31721003,32022024,32370842,32100633,32270850,32070652 and 81630035);the Shuguang Program of the Shanghai Education Development Foundation and Shanghai Municipal Education Commission(22SG20).

摘　　要：Naive pluripotent state can be obtained by several strategies from various types of cells,in which the cell fate roadmap as well as key biological events involved in the journey have been described in detail.Here,we carefully explored the chromatin accessibility dynamics during the primed-to-naïve transition by adopting a dual fluorescent reporter system and the assay for transposase-accessible chromatin(ATAC)-seq.Our results revealed critical chromatin remodeling events and highlight the discordance between chromatin accessibility and transcriptional activity.We further demonstrate that the differential epigenetic modifications and transcription factor(TF)activities may play a critical role in regulating gene expression,and account for the observed variations in gene expression despite similar chromatin landscapes.

关 键 词：Naive pluripotency Primed-to-naïve transition Dual reporter system Discordance between chromatin accessibility and transcriptional activity Histone modifications 

分 类 号：R318[医药卫生—生物医学工程]