Untargeted metabolomics to evaluate antifungal mechanism:a study of Cophinforma mamane and Candida albicans interaction  

作　　者：Asih Triastuti Marieke Vansteelandt Fatima Barakat Carlos Amasifuen Patricia Jargeat Mohamed Haddad 

机构地区：[1]UMR 152 Pharma Dev,IRD,UPS,Universitéde Toulouse,31400 Toulouse,France [2]Department of Pharmacy,Universitas Islam Indonesia,Yogyakarta 55584,Indonesia [3]Dirección de Recursos Genéticos y Biotecnología,Instituto Nacional de Innovación Agraria,Avenida La Molina 1981,La Molina,Lima 15024,Peru [4]Laboratoire Evolution et DiversitéBiologique UMR 5174,CNRS,IRD,UPS,Universitéde Toulouse,31062 Toulouse,France

出　　处：《Natural Products and Bioprospecting》2023年第1期779-787,共9页应用天然产物（英文）

摘　　要：Microbial interactions between filamentous fungi and yeast are still not fully understood.To evaluate a potential anti-fungal activity of a filamentous fungus while highlighting metabolomic changes,co-cultures between an endophytic strain of Cophinforma mamane(CM)and Candida albicans(CA)were performed.The liquid cultures were incubated under static conditions and metabolite alterations during the course were investigated by ultra-performance liquid chromatography-tandem mass spectrophotometry(UPLC-MS/MS).Results were analyzed using MS-DIAL,MS-FINDER,METLIN,Xcalibur,SciFinder,and MetaboAnalyst metabolomics platforms.The metabolites associated with catabolic processes,including the metabolism of branched-chain amino acids,carnitine,and phospholipids were upregulated both in the mono and co-cultures,indicating fungal adaptability to environmental stress.Several metab-olites,including C20 sphinganine 1-phosphate,myo-inositol,farnesol,gamma-undecalactone,folinic acid,palmitoleic acid,and MG(12:/0:0/0:0)were not produced by CA during co-culture with CM,demonstrating the antifungal mecha-nism of CM.Our results highlight the crucial roles of metabolomics studies to provide essential information regarding the antifungal mechanism of C.mamane against C.albicans,especially when the lost/undetected metabolites are involved in fungal survival and pathogenicity.

关 键 词：Metabolomics Fungal co-culture ANTI-FUNGAL VIRULENCE 

分 类 号：O62[理学—有机化学]