基于PB-MWCNTs-GNPs修饰的电化学免疫传感器检测流产布鲁氏菌  

Detection of Brucella abortus using an electrochemical immunosensor modified with PB-MWCNTs-GNPs

作　　者：迟宇 曹宇[1] 程浩 曹静文 敖建玥 李海波[2] 马雷[1,4] 刘明 CHI Yu;CAO Yu;CHENG Hao;CAO Jingwen;AO Jianyue;LI Haibo;MA Lei;LIU Ming(Clinical Medicine College,Jiamusi University,Jiamusi,Heilongjiang Province,154007;Faculty of Pharmacy and Laboratory Medicine,Army Medical University(Third Military Medical University),Chongqing,400038;Department of Clinical Laboratory,Banan Hospital Affiliated to Chongqing Medical University,Chongqing,401320;Department of Clinical Laboratory,Chenjiaqiao Hospital of Shapingba District,Chongqing,401331,China)

机构地区：[1]佳木斯大学临床医学院,黑龙江佳木斯154007 [2]陆军军医大学(第三军医大学)药学与检验医学系,重庆400038 [3]重庆医科大学附属巴南医院检验科,重庆401320 [4]重庆市沙坪坝区陈家桥医院检验科,重庆401331

出　　处：《陆军军医大学学报》2024年第17期1969-1975,共7页Journal of Army Medical University

摘　　要：目的构建一种高性能的电化学免疫传感器用于检测流产布鲁氏菌。方法制备普鲁士蓝(prussian blue,PB)-多壁碳纳米管(multi walled carbon nanotubes,MWCNTs)-纳米金粒子(gold nanoparticles,GNPs)纳米复合物,以流产布鲁氏菌作为待测样本,选择合适的抗体构建电化学免疫传感器,通过对传感器构建关键因素的考察确定最优条件,最后对传感器进行性能评价。结果明确传感器最优构建条件为:MWCNTs-PB混合比例为1∶5,材料干燥温度为37℃,缓冲体系最佳pH=7.5,抗体孵育时间为1 h,样本孵育时间为30 min;流产布鲁氏菌在10~1×10^(5) CFU/mL范围内呈良好的线性关系,传感器抗干扰能力、检测重复性与稳定性好,准确度高。结论基于PB-MWCNTs-GNPs纳米材料所修饰的用于检测流产布鲁氏菌的电化学免疫传感器构建简便,性能良好,可为布鲁氏病的临床早期诊断提供参考。Objective To prepare a high performance electrochemical immunosensor for detecting Brucella abortus(B.abortus).Methods Prussian blue(PB),multi walled carbon nanotubes(MWCNTs)and gold nanoparticles(GNPs)(PB-MWCNTs-GNPs)nanocomposites were prepared,and appropriate antibody was used to construct the immunosensor for detecting B.abortus samples.The optimal conditions were clarified by examining the key factors in sensor construction,and then the performance of the sensor was evaluated.Results The optimal construction conditions were determined as follows:the ratio of MWCNTs-PB was 1∶5,the drying temperature was 37℃,the pH value of buffer system was 7.5,and the incubation time of antibody and sample was 1 h and 30 min,respectively.B.abortus exhibited a good linear relationship,when ranging from 10 to 1×10^(5) CFU/mL.The sensor had good anti-interference ability,repeatability,stability and high accuracy.Conclusion Our prepared PB-MWCNTs GNPs nanomaterials modified electrochemical immunosensor for detecting B.abortus is easy to prepare,has good performance,and can provide reference for the early clinical diagnosis of brucellosis.

关 键 词：电化学免疫传感器 PB-MWCNTs-GNPs 流产布鲁氏菌 

分 类 号：R318.08[医药卫生—生物医学工程] R378.5[医药卫生—基础医学] R446.61