刚毛柽柳ThZFP3基因的耐盐抗旱功能  

Salt tolerance and drought resistance function of ThZFP3 gene in Tamarix hispida

作　　者：韩刚 贾园园 朱祯煜 赵鑫[1] 牛怡 王晓东 崔天翔 王超 HAN Gang;JIA Yuanyuan;ZHU Zhenyu;ZHAO Xin;NIU Yi;WANG Xiaodong;CUI Tianxiang;WANG Chao(College of Forestry,Northeast Forestry University,State Key Laboratory of Tree Genetics and Breeding,Harbin 150040,Heilongjiang,China)

机构地区：[1]东北林业大学林学院,林木遗传育种全国重点实验室,黑龙江哈尔滨150040

出　　处：《中南林业科技大学学报》2024年第8期139-149,共11页Journal of Central South University of Forestry & Technology

基　　金：国家自然科学基金项目(31870665);黑龙江省自然科学基金项目(LH2021C013)。

摘　　要：【目的】锌指蛋白(ZFP,Zinc Finger Proteins)是植物中的一类重要的转录调控因子,在植物生长发育、逆境胁迫应答等方面具有重要的作用。通过分析刚毛柽柳Tamarix hispida锌指蛋白基因ThZFP3的耐盐、抗旱功能,为林木抗逆遗传改良提供了理论基础。【方法】在通过蘸花法获得过表达ThZFP3转基因拟南芥;在NaCl和Mannitol(甘露醇)胁迫下,观察转基因和对照拟南芥的萌芽成活率、根长、鲜质量和表型变化;在利用农杆菌瞬时转化体系获得瞬时过表达(OE)、抑制表达(RNAi)和对照(Control)转基因刚毛柽柳,胁迫下分别对三种转基因柽柳进行组织化学染色和抗逆相关生理指标测定,鉴定ThZFP3基因的抗旱、耐盐能力。【结果】在胁迫条件下,转基因拟南芥的萌芽成活率、根长、鲜质量和长势均优于野生型,表明ThZFP3基因可提高转基因拟南芥的抗旱耐盐能力;过表达ThZFP3基因显著增强了刚毛柽柳中POD和SOD的活性,从而减少活性氧(ROS)的积累;过表达ThZFP3基因可以减少刚毛柽柳中MDA的积累、降低电解质渗透率,保护细胞膜的完整。【结论】过表达ThZFP3基因可以提高刚毛柽柳体内ROS清除能力,降低细胞损伤程度,从而提高刚毛柽柳的抗旱耐盐能力。【Objective】Zinc finger proteins are a major class of important transcription regulatory factors in plants,playing important roles in plant growth and development,and stress response.The salt tolerance and drought resistance function of ThZFP3 of Tamarix hispida zinc finger protein gene was analyzed to provide a theoretical basis for genetic improvement of tree resistance.【Method】Arabidopsis thaliana was transformed with ThZFP3 using the floral dip method.The germination survival rate,root length,fresh mass,and phenotypic changes of the transgenic and control A.thaliana were observed under NaCl and mannitol stress.Transient overexpression(OE),inhibited expression(RNAi)and control transgenic Tamarix were obtained using the Agrobacterium transient transformation system.Three transgenic Tamarix plants were histochemically stained and stress-related physiological indexes were measured to identify the drought resistance and salt tolerance of the ThZFP3 gene.【Result】Under stress conditions,the germination survival rate,root length,fresh mass,and growth vigor of transgenic Arabidopsis were superior to wild-type strains,indicating that the ThZFP3 gene improved the drought and salt tolerance of transgenic Arabidopsis.Overexpression of the ThZFP3 gene significantly enhanced the activities of POD and SOD in T.hispida,thereby reducing the accumulation of reactive oxygen species(ROS);Overexpression of the ThZFP3 gene reduced the accumulation of MDA and electrolyte permeability in T.hispida,protecting the integrity of cell membranes.【Conclusion】Overexpression of the ThZFP3 gene can improve the ROS scavenging ability within the body of T.hispida,reduce the degree of cell damage,and thus improve its drought resistance and salt tolerance.

关 键 词：刚毛柽柳 CCCH型锌指蛋白 耐盐 抗旱 

分 类 号：S718.43[农业科学—林学]