Multi‑integrated genomic data for Passiflora foetida provides insights into genome size evolution and floral development in Passiflora  

作　　者：Yi Zou Jie Wang Dan Peng Xiaoni Zhang Luke R.Tembrock Jinliang Yang Jianli Zhao Hong Liao Zhiqiang Wu 

机构地区：[1]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,China [2]Ministry of Education Key Laboratory for Transboundary Ecosecurity of Southwest China,Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology,Institute of Biodiversity,School of Ecology and Environmental Science,Yunnan University,Kunming,Yunnan 650504,China [3]College of Agriculture,Center for Genomics and Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China [4]Department of Agricultural Biology,Colorado State University,Fort Collins,CO 80523,USA [5]Department of Agronomy and Horticulture,University of Nebraska-Lincoln,Lincoln,NE 68583,USA [6]Kunpeng Institute of Modern Agriculture at Foshan,Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518124,China

出　　处：《Molecular Horticulture》2023年第1期19-38,共20页分子园艺（英文）

基　　金：funded by the Guangdong Pearl River Talent Program(grants 2021QN02N792);the Shenzhen Fundamental Research Program(grants JCYJ20220818103212025);the Science,Technology and Innovation Commission of Shenzhen Municipality(STIC:RCYX20200714114538196);the Scientific Research Foundation for Principle Investigator from Kunpeng Institute of Modern Agriculture at Foshan(grants KIMA-QD2022004);the Chinese Academy of Agricultural Sciences Elite Youth Program(grants 110243160001007);the National Natural Science Foundation of China(32100184);the Grants from Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology(YNPRAEC-2023001).

摘　　要：Passiflora is a plant genus known for its extremely distinctive and colorful flowers and a wide range of genome size variation.However,how genome characteristics are related to flower traits among Passiflora species remains poorly understood.Here,we assembled a chromosome-scale genome of P.foetida,which belongs to the same subgenus as the commercial passionfruit P.edulis.The genome of P.foetida is smaller(424.16 Mb)and contains fewer copies of long terminal repeat retrotransposons(LTR-RTs).The disparity in LTR-RTs is one of the main contributors to the differences in genome sizes between these two species and possibly in floral traits.Additionally,we observed variation in insertion times and copy numbers of LTR-RTs across different transposable element(TE)lineages.Then,by integrating transcriptomic data from 33 samples(eight floral organs and flower buds at three developmental stages)with phylogenomic and metabolomic data,we conducted an in-depth analysis of the expression,phylogeny,and copy number of MIKC-type MADS-box genes and identified essential biosynthetic genes responsible for flower color and scent from glandular bracts and other floral organs.Our study pinpoints LRT-RTs as an important player in genome size variation in Passiflora species and provides insights into future genetic improvement.

关 键 词：Passiflora foetida Genome assembly Transposable elements MADS-BOX Floral organ 

分 类 号：Q78[生物学—分子生物学]