Function and molecular mechanism analysis of CaLasSDE460 effector involved in the pathogenesis of“Candidatus Liberibacter asiaticus”in citrus  

作　　者：Shuai Wang Meixia Du Liting Dong Rongrong Qu Danlu Ran Juanjuan Ma Xuefeng Wang Lanzhen Xu Weimin Li Yongrui He Xiuping Zou 

机构地区：[1]Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City,Citrus Research Institute,Southwest University/National Citrus Engineering Research Center,Chongqing,People’s Republic of China [2]Key Laboratory for Northern Urban,Agriculture of Ministry of Agriculture and Rural Affairs,Beijing University of Agriculture,Beijing,People’s Republic of China

出　　处：《Molecular Horticulture》2023年第1期197-213,共17页分子园艺（英文）

基　　金：Open access funding provided by Shanghai Jiao Tong University;supported by the National Key Research and Development Program of China(2021YFD1400800);the National Natural Sciences Foundation of China(31972393);the Earmarked Fund for China Agriculture Research System(CARS-27).

摘　　要：Citrus Huanglongbing(HLB),caused by Candidatus Liberibacter asiaticus(CaLas),is the most serious disease worldwide.CaLasSDE460 was previously characterized as a potential virulence factor of CaLas.However,the function and mechanism of CaLasSDE460 involved in CaLas against citrus is still elusive.Here,we showed that transgenic expression of CaLasSDE460 in Wanjincheng oranges(C.sinensis Osbeck)contributed to the early growth of CaLas and the development of symptoms.When the temperature increased from 25℃to 32℃,CaLas growth and symptom development in transgenic plants were slower than those in WT controls.RNA-seq analysis of transgenic plants showed that CaLasSDE460 affected multiple biological processes.At 25℃,transcription activities of the“Protein processing in endoplasmic reticulum”and“Cyanoamino acid metabolism”pathways increased while transcription activities of many pathways decreased at 32℃.124 and 53 genes,separately annotated to plant-pathogen interaction and MAPK signaling pathways,showed decreased expression at 32℃,compared with these(38 for plant-pathogen interaction and 17 for MAPK signaling)at 25℃.Several important genes(MAPKKK14,HSP70b,NCED3 and WRKY33),remarkably affected by CaLasSDE460,were identified.Totally,our data suggested that CaLasSDE460 participated in the pathogenesis of CaLas through interfering transcription activities of citrus defense response and this interfering was temperature-dependent.

关 键 词：CITRUS HLB CaLasSDE460 TRANSCRIPTOME Ectopic expression 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]