Evolutionary assessment of SQUAMOSA PROMOTER BINDING PROTEIN‑LIKE genes in citrus relatives with a specific focus on flowering  

作　　者：Yawei Li Shuting Wang Prakash Babu Adhikari Bing Liu Shengjun Liu Yue Huang Gang Hu Michitaka Notaguchi Qiang Xu 

机构地区：[1]National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops,Huazhong Agricultural University,Wuhan 430000,China [2]Bioscience and Biotechnology Center,Nagoya University,Furo‑cho,Chikusa‑ku,Nagoya 464‑8601,Japan [3]Hubei Hongshan Laboratory,Wuhan 430070,China

出　　处：《Molecular Horticulture》2023年第1期214-229,共16页分子园艺（英文）

基　　金：Open access funding provided by Shanghai Jiao Tong University;supported by Major Special Projects and Key R&D Projects in Yunnan Province(NO.202102AE090054);the National Natural Science Foundation of China granted to Q.X.(numbers 31925034);Key project of Hubei provincial Natural Science Foundation(2021CFA017).

摘　　要：Phase transition and floral induction in citrus requires several years of juvenility after germination.Such a long period of juvenility has been a major hindrance to its genetic improvement program.Studies have shown that miR156 along with its downstream genes SQUAMOSA PROMOTER BINDING PROTEINS(SBP)and SBP-LIKE(SPL)mediate the phase transition and floral induction process in plants.Our current study has systematically analyzed SPLs in 15 different citrus-related species,systematically annotated them based on their close homology to their respective Arabidopsis orthologs,and confirmed the functional attributes of the selected members in floral precocity.The majority of the species harbored 15 SPLs.Their cis-element assessment suggested the involvement of the SPLs in diverse developmental and physiological processes in response to different biotic and abiotic cues.Among all,SPL5,SPL9,and SPL11 stood out as consistently differentially expressed SPLs in the adult and young tissues of different citrusrelated species.Independent overexpression of their F.hindsii orthologs(FhSPL5,FhSPL9,and FhSPL11)brought an enhanced expression of endogenous FLOWERING LOCUS T leading to the significantly precocious flowering in transgenic Arabidopsis lines.Future study of the genes in the citrus plant itself is expected to conclude the assessments made in the current study.

关 键 词：SPL Gene Family PHYLOGENY Phase Transition Early Flowering 

分 类 号：Q94[生物学—植物学]