MALDI-TOF MS法快速鉴定江苏口岸输入性蜚蠊携带致病菌  

Rapid Identification of Pathogenic Bacteria Carried by Imported Cockroaches at Jiangsu port Using MALDI-TOF MS Method

作　　者：孟媛 苏小建 杨国平 蔡丽萍 张荣 易海华 田锋 耿合员 MENG Yuan;SU Xiao-Jian;YANG Guo-Ping;CAI Li-Ping;ZHANG Rong;YI Hai-Hua;TIAN Feng;GENG He-Yuan(Jiangsu International Travel Health Care Center(Nanjing Customs Port Outpatient Department),Nanjing 210019;Changshu Customs Comprehensive Technical Service Center,Changshu 215500;Xinjiang International Travel Health Care Center(Urumqi Customs Port Outpatient Department),Urumqi 830011)

机构地区：[1]江苏国际旅行卫生保健中心(南京海关口岸门诊部),南京210019 [2]常熟海关综合技术服务中心,常熟215500 [3]新疆国际旅行卫生保健中心(乌鲁木齐海关口岸门诊部),乌鲁木齐830011

出　　处：《中国口岸科学技术》2024年第8期13-17,共5页China Port Science and Technology

基　　金：国家重点研发计划(2021YFC2301200);海关总署科研项目(2023HK03);南京海关科研项目(2022KJ15,2023KJ02)。

摘　　要：本文旨在探索利用飞行时间质谱MALDI-TOF MS快速鉴定江苏口岸输入性病媒生物蜚蠊携带致病菌种类特征,为防控蜚蠊携带致病菌提供技术指导。通过将口岸截获的输入性病媒生物蜚蠊组织研磨后进行细菌培养,对单一菌落分别通过基质辅助激光解吸电离飞行时间质谱(Matrix-Assisted Laser Desorption/Ionization Time of Flight,MALDI-TOF MS)法进行鉴定,以检测蜚蠊携带致病菌种类特征。结果显示,通过MALDI-TOF MS法鉴定出蜚蠊携带的菌群主要是革兰阴性杆菌,包括肺炎克雷伯菌(Klebsiella pneumoniae,KPN)、产酸克雷伯菌(Klebsiella oxytoca,Kox)、铜绿假单胞菌(Pseudomonas aeruginosa,PAE)、大肠埃希菌(Escherichia coli,E.coli)、奇异变形杆菌(Proteus mirabilis)及其亚型等。少数为携带革兰阳性菌,包括金黄色葡萄球菌(Staphylococcus aureus,MRSA)及其他革兰阳性葡萄球菌的亚型、蜡样芽孢杆菌(Bacillus cereus)及其他芽孢杆菌等。综上,MALDI-TOF-MS法可实现对口岸输入性病媒生物蜚蠊携带致病菌的快速鉴定,为口岸输入性蜚蠊携带传播疾病的防控提供技术支持。This study aims to explore the rapid identification of the pathogenic bacteria carried by cockroaches,an imported vector species at Jiangsu ports,using Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry(MALDI-TOF MS),to provide technical guidance for the prevention and control of cockroaches.By grinding the tissues of intercepted imported vector cockroaches and culturing the bacteria,we identified individual colonies using MALDI-TOF MS to detect the species characteristics of pathogenic bacteria carried by cockroaches.According to the result of MALDI-TOF MS,the main bacteria carried by cockroaches were Gram-negative bacilli,including Klebsiella pneumoniae(KPN),Klebsiella oxytoca(Kox),Pseudomonas aeruginosa(PAE),Escherichia coli(E.coli),Proteus mirabilis and their subtypes.A minority were Gram-positive bacteria,including Staphylococcus aureus(MRSA)and other Gram-positive staphylococcus subtypes,Bacillus cereus and other bacillus species.These findings indicate that MALDI-TOF-MS method can achieve rapid identification of pathogenic bacteria carried by imported vector-borne cockroaches at ports,and provide technical support for the prevention and control of imported vector-borne diseases.

关 键 词：基质辅助激光解吸电离飞行时间质谱 蜚蠊 口岸输入 致病菌 

分 类 号：R185[医药卫生—流行病学]