全自动血型分析系统检测Kk血型抗原方法的建立与验证  

Establishment and Validation of a Method for Detecting Kk Blood Group Antigen Using a Fully Automated Blood Group Analysis System

作　　者：马维娟 杨忠思 潘海平 于琦 MA Weijuan;YANG Zhongsi;PAN Haiping;YU Qi(Department of Quality Management,Qingdao Blood Center,Qingdao Shandong 266071,China)

机构地区：[1]青岛市中心血站质量管理科,山东青岛266071

出　　处：《中国卫生标准管理》2024年第16期142-145,共4页China Health Standard Management

摘　　要：目的针对BECKMAN PK7300全自动血型分析系统检测Kk血型抗原建立非配套检测方法并进行验证,为后期常规开展Kk抗原检测提供基础数据支持。方法随机抽取青岛市中心血站2021年11月—2022年4月的40名无偿献血者样本。基于相关文献查阅梳理结果,优化试验参数选择孵育温度、试剂加注量、稀释标本加注量,根据设备厂家推荐参数评价验证样本红细胞的稀释浓度、抗-K和抗-k血清的稀释浓度对样本检测的影响。结果利用PK7300全自动血型分析仪进行非配套检测Kk抗原后得到的各项参数为样本红细胞和抗-K、抗-k血清的稀释浓度分别为1.50%和1∶20;孵育温度和时间分别为30℃和1 h;试剂和样本的反应体积相同,均为25μL。结论针对Kk抗原检测建立的PK7300非配套血型检测系统得出的实验参数特异性好、敏感度高,符合实验预期。经过对样本及试剂稀释倍数的检测验证,有助于本研究建立的检测系统进行稀有血型批量检测准确判读和检测效率的提升,同时可降低试剂损耗。Objective The non-supporting detection methods for Kk blood group antigen detection by BECKMAN PK7300 automatic blood group analysis system were established and verified,providing basic data support for routine Kk antigen detection in the later stage.Methods A total of 40 unpaid blood donors from Qingdao Blood Center from November 2021 to April 2022 were randomly selected.Based on the results of literature review,the experiment parameters were optimized to select incubation temperature,reagent dosage and dilution specimen dosage.According to the recommended parameters of the equipment manufacturer,the influence of the diluted concentration of red blood cells,anti-K and anti-K serum on the sample detection was evaluated.Results The parameters of Kk antigen were obtained by using PK7300 automatic blood group analyzer.The diluted concentrations of red blood cells and anti-K and anti-K serum were 1.50%and 1:20,respectively.The incubation temperature and time were 30℃and 1 h.The reaction volume of the reagent and sample was the same,both of which were 25μL.Conclusion The experimental parameters of PK7300 non-matching blood group detection system established for Kk antigen detection have good specificity and high sensitivity,which are in line with the experimental expectation.The detection and verification of samples and reagent dilution ratio will help the detection system established in this study to accurately interpret the rare blood group batch detection and improve the detection efficiency,while reducing the loss of reagents.

关 键 词：血型 全自动血型分析仪 微板法 Kk抗原 红细胞 无偿献血者 

分 类 号：R457[医药卫生—治疗学]