家畜血液储存条件及预处理方式对种属鉴定的影响  

The influence of blood storage conditions and pretreatment methods on species identification in livestock

作　　者：王欣 董蕾 汪雅雯 WANG Xin;DONG Lei;WANG Yawen(SPH NO.1 Biochemical&Pharmaceutical Co.,Ltd.,Shanghai 200240,China)

机构地区：[1]上海上药第一生化药业有限公司,上海200240

出　　处：《上海医药》2024年第15期87-90,共4页Shanghai Medical & Pharmaceutical Journal

摘　　要：目的:考察血液储存时间、储存温度和预处理方式对种属鉴定的影响。方法:以猪、牛、羊血液为样本,考察储存时间、储存温度和预处理方式对种属鉴定的影响。储存时间考察1周和3周,储存温度考察4℃和−20℃,预处理方式考察是否去除红细胞。样本经DNA提取,PCR-RFLP检测后进行结果判定。结果与结论:储存1周时,两种储存温度及预处理方式均能正常进行种属鉴定。储存3周时,两种储存温度,预处理时不去除红细胞会产生假阴性判定,假阴性是由于提取的DNA中存在PCR抑制物;预处理时去除红细胞可正常检测。该结论对于猪、牛、羊血液具有普适性。Objective:To investigate the effects of different storage time,temperature and pretreatment methods on the results of blood species identification.Methods:The effects of storage time(one or three weeks)and temperature(4 or−20℃)and pretreatment methods(removing red blood cells or not)on species identification were investigated taking the blood of pig,bovine and sheep for samples.The samples were subjected to DNA extraction and PCR-RFLP detection for result determination.Results&Conclusion:The samples could be used for normal species identification when stored for one week and at 4 or−20℃and pretreated by removing red blood cells or not.The false-negative verdicts were generated when samples were stored for 3 weeks at either 4 or−20℃and the red blood cells were not removed during pretreatment,which may be due to the presence of PCR inhibitors in the extracted DNA.The species identification could be normally performed by removing red blood cells during pretreatment.This conclusion has general applicability to pig,bovine and sheep blood.

关 键 词：血液 种属鉴定 PCR 抑制物 

分 类 号：TQ464.8[化学工程—制药化工] TQ460.4