抗丁醚脲二斑叶螨种群筛选及生化抗性机理初探  

Screening of Tetranychus urticae populations for resistance to diafenthiuron and the mechanism of biochemical resistance to this pesticide

作　　者：叶调琴 杨顺义 李文平 YE Tiao-Qin;YANG Shun-Yi;LI Wen-Ping(College of Plant Protection,Gansu Agricultural University,Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学植物保护学院,甘肃省农作物病虫害生物防治工程实验室,兰州730070

出　　处：《应用昆虫学报》2024年第3期514-521,共8页Chinese Journal of Applied Entomology

基　　金：甘肃农业大学学科建设基金(GAU-XKJS-2018-07)。

摘　　要：【目的】探究二斑叶螨Tetranychus urticae对丁醚脲的抗性发展情况及生化抗性机理,以期为二斑叶螨的抗药性治理提供理论依据。【方法】采用室内生物测定,用丁醚脲对二斑叶螨进行持续抗性选育33代;用生化分析方法,每隔6代测定二斑叶螨丁醚脲抗性种群羧酸酯酶(Carboxylesterase,CarEs)、谷胱甘肽S-转移酶(Glutathione S-transferase,GSTs)、多功能氧化酶(Mixed-function oxidases,MFOs)和几丁质酶(Chitinases)的活力变化情况。【结果】二斑叶螨经丁醚脲汰选至33代时,抗性指数达到18.610倍;抗性种群体内CarEs、GSTs和MFOs活性较敏感种群显著提高(P<0.05),其相对倍数分别为1.909、2.119和7.436倍,同时几丁质酶的活性较敏感种群无显著差异(P>0.05),其相对倍数为0.594。【结论】二斑叶螨对丁醚脲的抗性主要与MFOs活性升高和几丁质酶活性降低有关,其次,GSTs和CarEs活性升高也参与了丁醚脲抗性的形成。[Aim]To investigate the development of resistance to diafenthiuron and the mechanism responsible for this resistance,in Tetranychus urticae.[Methods]A susceptible population of T.urticae was continuously selected for resistance to diafenthiuron for 33 generations in a laboratory.The activity of carboxylesterases(CarEs),glutathione S-transferases(GSTs),Mixed-function oxidases(MFOs)and chitinases(chitinases)in this population was tested every six generations.[Results]The resistance index had reached 18.61 after 33 generations.The activity of CarEs,GSTs and MFOs in the resistant population were significantly higher(1.909,2.119 and 7.436-fold,respectively)than in the sensitive population(P<0.05).However,there was no significant difference in the chitinase activity in the sensitive populations(0.594,P<0.05).[Conclusion]Resistance of T.urticae to diafenthiuron is mainly associated with MFOs and chitinase activity,although GSTs and CarEs also play a role.

关 键 词：二斑叶螨 丁醚脲 抗性培育 解毒酶 几丁质酶 

分 类 号：S433[农业科学—农业昆虫与害虫防治]