MP感染合并哮喘患儿血清miR-424-5p、MCP-1、TLR4水平联合检测预测预后的效能  

Efficacy of combined detection of serum levels of miR-424-5p,MCP-1,and TLR4 in predicting the prognosis of children with Mycoplasma pneumoniae infection and asthma

作　　者：卓艳霞 张敬芳[1] 张永卓[1] 孙明昌[1] 孙明华[1] 张俊霞[1] ZHUO Yan-xia;ZHANG Jing-fang;ZHANG Yong-zhuo;SUN Ming-chang;SUN Ming-hua;ZHANG Jun-xia(Department of Pediatrics,Puyang People's Hospital,Puyang 457000,Henan,CHINA)

机构地区：[1]濮阳市人民医院儿科,河南濮阳457000

出　　处：《海南医学》2024年第17期2496-2500,共5页Hainan Medical Journal

基　　金：2022年河南省医学科技攻关联合共建项目(编号:LHGJ20221014)。

摘　　要：目的探讨肺炎支原体(MP)感染合并哮喘患儿血清miR-424-5p、单核细胞趋化蛋白-1(MCP-1)、Toll样受体4(TLR4)水平联合检测预测预后的效能。方法回顾性选取2021年8月至2023年8月濮阳市人民医院收治的107例MP感染合并哮喘患儿作为研究对象,所有患儿均给予对症治疗,根据6个月预后分为预后良好组86例和预后不良组21例。比较两组患儿的基线资料和血清miR-424-5p、MCP-1、TLR4水平,采用Logistic回归分析各血清指标对预后的影响,绘制受试者工作特征(ROC)曲线及曲线下面积(AUC)分析血清miR-424-5p、MCP-1、TLR4对预后的预测效能。结果两组患儿的病程、发热天数、呼吸道感染史比较差异均有统计学意义(P<0.05);预后不良组患儿的血清miR-424-5p、MCP-1、TLR4水平分别为1.56±0.41、(84.29±15.19)μg/L、(95.21±13.52)ng/mL,明显高于预后良好组的1.23±0.33、(70.38±11.36)μg/L、(83.10±10.17)ng/mL,差异均有统计学意义(P<0.05);校正前Logistic回归分析结果显示,病程、发热天数、呼吸道感染史、血清miR-424-5p、MCP-1、TLR4均是MP感染合并哮喘患儿预后的独立影响因素(P<0.05),校正后Logistic回归分析结果显示,血清miR-424-5p、MCP-1、TLR4仍是MP感染合并哮喘患儿预后的独立影响因素(P<0.05);ROC曲线结果显示,血清miR-424-5p、MCP-1、TLR4联合检测预测MP感染合并哮喘患儿预后的AUC为0.900,优于各血清指标单独预测。结论MP感染合并哮喘患儿血清mi R-424-5p、MCP-1、TLR4与预后密切相关,三者联合检测预测MP感染合并哮喘患儿预后具有良好的参考价值。Objective To explore the prognostic efficacy of combined detection of serum miR-424-5p,mono-cyte chemoattractant protein-1(MCP-1),and toll-like receptor 4(TLR4)levels in children with Mycoplasma pneumoniae(MP)infection and asthma.Methods A retrospective study was conducted on 107 children with MP infection and asth-ma admitted to Puyang People's Hospital from August 2021 to August 2023.All children were given symptomatic treat-ment,and they were divided into a good prognosis group(86 cases)and a poor prognosis group(21 cases)based on their prognosis at 6 months.The baseline data and serum levels of miR-424-5p,MCP-1,and TLR4 were compared between the two groups of children,and logistic regression analysis was used to analyze the impact of each serum indicator on prognosis.The receiver operating characteristic(ROC)curve and area under the curve(AUC)were used to analyze the predictive efficacy of serum miR-424-5p,MCP-1,and TLR4 on prognosis.Results There were significant differences in the duration of illness,days of fever,and history of respiratory tract infection between the two groups(P<0.05).The levels of serum miR-424-5p,MCP-1,and TLR4 in poor prognosis group were 1.56±0.41,(84.29±15.19)μg/L,and(95.21±13.52)ng/mL,respectively,which were significantly higher than 1.23±0.33,(70.38±11.36)μg/L,and(83.10±10.17)ng/mL in good prognosis group,respectively(P<0.05).The results of logistic regression analysis before adjust-ment showed that the course of disease,days of fever,history of respiratory tract infection,serum miR-424-5p,MCP-1,and TLR4 were all independent factors influencing the prognosis of children with MP infection complicated with asthma(P<0.05),and the results of logistic regression analysis after adjustment showed that miR-424-5p,MCP-1,and TLR4 were still independent factors affecting the prognosis of children with MP infection and asthma(P<0.05).The ROC curve showed that the AUC of the combined detection of serum miR-424-5p,MCP-1,and TLR4 for predicting the prog-nosis of children with MP infection

关 键 词：肺炎支原体 哮喘 miR-424-5p 单核细胞趋化蛋白-1 Toll样受体4 预后 

分 类 号：R725.6[医药卫生—儿科]