蓖麻油体蛋白基因家族鉴定和盐碱胁迫响应分析  

Identification for Oleosin Gene Family and Analysis on Its Saline and Alkaline Stress Response in Ricinus communis L.

作　　者：何汉琼 姜洪进 张文婧 马钰雯 孟子轩 李月明 邓晓霞 蔺吉祥 HE Hanqiong;JIANG Hongjin;ZHANG Wenjing;MA Yuwen;MENG Zixuan;LI Yueming;DENG Xiaoxia;LIN Jixiang(College of Agricultural Environment and Resources,Shanxi Agricultural University,Taiyuan 030031,China;College of Landscape Architecture,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]山西农业大学资源环境学院,太原030031 [2]东北林业大学园林学院,哈尔滨150040

出　　处：《种子》2024年第8期103-111,共9页Seed

基　　金：山西农业大学校科技创新提升工程项目(CXGC2023027)。

摘　　要：本研究以蓖麻为对象,利用生物信息学手段对蓖麻Oleosin基因家族进行全面鉴定分析,得到5个Oleosin基因家族成员,序列长度在138~232 aa之间;分子量在14.48493~25.42461 kDa之间;等电点介于9.07~10.96之间;均为疏水性蛋白且具有高度保守的“脯氨酸结”;α-螺旋和无规则卷曲是蓖麻Oleosin蛋白的主要组成结构;基序分析显示,Motif1~Motif4存在所有蓖麻Oleosin蛋白中。编码这些蛋白的5个Oleosin基因分布于4条染色体上,在进化关系上被划分到U-Oleosin、SL-Oleosin和SH-Oleosin三个亚族;基因结构分析显示,除RcOleosin2有一个内含子外,其余RcOleosin基因均无内含子;5个基因启动子区域均存在多种与逆境响应相关的顺式作用元件;共线性分析显示,基因组多倍化不是蓖麻Oleosin基因家族扩张的主要原因;盐碱胁迫下的蓖麻种子转录组数据表明,5个Oleosin基因均在盐碱胁迫过程中有一定的调节功能。In this study,using Ricinus communis L.as research object,the Ricinus communis L.Oleosin gene family was comprehensively identified and analyzed by bioinformatics methods.Five members of Oleosin gene family with sequence length ranging from 138 to 232 aa were obtained.The molecular weight ranged from 14.48493 to 25.42461 kDa.The isoelectric point was between 9.07 and 10.96;They were hydrophobic proteins with highly conserved proline junctions.α-helix and random curling were the main components of Ricinus communis L.Oleosin.Motif1 to Motif4 were found in all Ricinus communis L.Oleosin proteins by motif analysis.The five Oleosin genes encoding these proteins were distributed on four chromosomes and were divided into three subfamilies,U-Oleosin,SLOleosin and SH-Oleosin.Gene structure analysis showed that all RcOleosin genes had no intron except RcOleosin2.There were multiple cis-acting elements related to stress response in all five gene promoter regions.Collinear analysis showed that genome polyploidy was not the main reason for the expansion of Ricinus communis L.Oleosin gene family.The transcriptome data of Ricinus communis L.seeds under salt-alkali stress showed that all five Oleosin genes had certain regulatory functions during salt-alkali stress.

关 键 词：蓖麻 油体蛋白 基因家族 生物信息学 

分 类 号：S565.6[农业科学—作物学]