长穗偃麦草 ThPLA 基因的电子克隆和生物信息学分析  

Electronic Cloning and Bioinformatics Analysis of ThPLA Genein Thinopyrum elongatum

作　　者：张宴萍 黄德华 李鹏[1] 崔正勇[1] 何方 闫百蔷 ZHANG Yanping;HUANG Dehua;LI Peng;CUI Zhengyong;HE Fang;YAN Baiqiang(Shandong Academy of Agricultural Sciences,National Engineering Research Center for Wheat and Maize,Ji'nan 250100,China;Shandong Institute of Innovation and Development,Ji'nan 250101,China;College of Agriculture,Guizhou Unversity,Guiyang 550025,China)

机构地区：[1]山东省农业科学院/小麦玉米国家工程研究中心,济南250100 [2]山东省创新发展研究院,济南250101 [3]贵州大学农学院,贵阳550025

出　　处：《种子》2024年第8期112-118,共7页Seed

基　　金：山东省农业科学院农业科技创新工程人才类“333工程”计划(CXGC2022E01);“十四五”重点研发计划(2022YFD1200701)。

摘　　要：本研究基于长穗偃麦草高通量测序信息,利用电子克隆方法获得长穗偃麦草磷脂酶基因(ThPLA),结合生物信息学分析方法,从氨基酸理化性质、保守结构域、高级结构、亲水性/疏水性、跨膜结构域、同源性分析及功能表达等方面对该基因gDNA和编码蛋白进行了预测和分析。结果表明,ThPLA基因全长1620 bp,编码539个氨基酸;与粗山羊草、野生二粒小麦、水稻和玉米的PLA蛋白亲缘关系最近;亚细胞定位和GO term预测ThPLA蛋白质位于叶绿体;综合基因表达预测和转录组数据结果,该基因可能在植物根部对盐胁迫做出响应。In this study,the phospholipase gene(ThPLA)of Thinopyrum elongatum was obtained by electronic cloning method based on high-throughput sequencing information.Combined with bioinformatics analysis methods,the amino acid physicochemical properties,conserved domain,advanced structure,hydrophilic/hydrophobic,transmembrane domain,homology analysis and functional expression of the gene gDNA and coding protein were predicted and analyzed.It was found that the total length of ThPLA gene was 1620 bp,encoding 539 amino acids,of which the PLA protein was most closely related to Aegilops tauschii Coss.,Triticum turgidum var.dicoccoides,Oryza sativa L.and Zea mays L..Subcellular localization and GO term predicted that ThPLA protein was located in chloroplasts.Combining the results of gene expression prediction and transcriptome data,the gene could respond to salt stress in plant roots.

关 键 词：长穗偃麦草 ThPLA基因 电子克隆 生物信息学 

分 类 号：S543[农业科学—作物学]