新疆小麦地方品种重要功能基因的KASP标记检测  

Detection of Important Functional Genes in Xinjiang Local Wheat Cultivars by KASP Markers

作　　者：哈力旦·依克热木 刘娜[1,2,3] 刘联正 周安定[1,2,3] 曹俊梅 张新忠[1,2,3] Halidan·Yikeremu;LIU Na;LIU Lianzheng;ZHOU Anding;CAO Junmei;ZHANG Xinzhong(Food Crop Research Institute,Xinjiang Academy of Agricultural Sciences,Urumuqi 830091,China;Northwest Wheat Drought and Salt Tolerance Biological Observation Station,Ministry of Agriculture and Rural Affairs,Urumuqi 830091,China;Urumqi Experimental Station of Xinjiang Wheat Industry Technology System,Urumuqi 830091,China)

机构地区：[1]新疆农业科学院粮食作物研究所,乌鲁木齐830091 [2]农业农村部西北地区小麦抗旱耐盐生物学科学观测实验站,乌鲁木齐830091 [3]新疆小麦产业技术体系乌鲁木齐综合试验站,乌鲁木齐830091

出　　处：《种子》2024年第8期126-133,138,共9页Seed

基　　金：新疆维吾尔自治区重大科技专项项目(2022A02003-1,2022A03004-3,2021A02001-1);新疆维吾尔自治区自然科学基金面上项目(2023D01A94);科技创新2030-重大项目子课题(2023ZD0402602);农业农村部盐碱土改良与利用(干旱半干旱区盐碱地)重点实验室基金课题(YJDKFJJ202304);农业科技创新稳定支持项目(xjnkywdzc-2023001-24);自治区公益性科研院所基本科研业务经费资助项目(KY2023029);新疆农业科学院青年科技骨干创新能力培养项目(xjnkq-2023001)。

摘　　要：本研究利用10种与表型性状有关的44个高通量KASP(Kompetitive allele specific PCR)分型标记技术,检测了117份新疆小麦地方品种的表型、品质、面粉色泽、籽粒蛋白质含量、粒重、抗逆、抗病等相关基因。结果表明,Ppd-D1光周期位点检测到非敏感材料116份。春化、矮杆基因位点检测中,冬性材料比例97%以上,矮化基因比例96%以上。Glu-A3和Glu-B3的标记检测中,高筋材料的比例仅占1%。有利等位基因Psy-B1中被检测出低黄色素材料,占95.7%;Pds-B1和Psy-A1基因中,以高黄色素材料为主。NAMA1检测出品质相关A1b、A1d类型材料116份。GCP_DUP标记鉴定携带低分子量谷蛋白亚基的材料占97%以上。抗病相关基因中,以抗叶锈病的材料为主。113份材料检测为抗穗发芽类型。This study using 10 kinds of phenotypic traits about 44 high-throughput KASP(kompetitive allele specific PCR)type markers,the phenotype,quality,flour colour and lustre,grain protein content,grain weight,stress resistance,disease resistance genes of 117 Xinjiang local wheat(Triticum aestivum L.)cultivars were tested.The results showed that 116 non-sensitive materials were detected at the photoperiod site of Ppd-D1.In the detection of vernalization and dwarf gene loci,the proportion of winter materials was more than 97%,and the proportion of dwarfing genes was more than 96%.In the detection of Glu-A3 and Glu-B3 labeling,the proportion of high reinforcement materials accounted for only 1%.Low yellow pigment material was detected in the favorable allele Psy-B1,accounting for 95.7%;Pds-B1 and Psy-A1 genes were mainly high yellow pigment materials.NAM-A1 detected 116 quality related A1b and A1d materials.More than 97%of materials carrying low molecular weight glutenin subunits were identified by GCP_DUP labeling.Among the genes related to disease resistance,the materials resistant to leaf rust were the main ones.113 materials were found to be anti-sprouting types.

关 键 词：小麦地方品种 功能基因 KASP标记 

分 类 号：S512.1[农业科学—作物学]