大花黄牡丹花粉活力检测方法筛选  

Screening of the Methods for Detecting the Pollen Viability of Paeonia ludlowii

作　　者：唐英 方亮媛 许建昌 王忠斌[1] 邢震[1] TANG Ying;FANG Liangyuan;XU Jianchang;WANG Zhongbin;XING Zhen(Xizang Agriculture and Animal Husbandry University,Nyingchi Xizang 860000,China;Nimu Natural Resources Bureau,Nimu Xizang 851300,China)

机构地区：[1]西藏农牧学院资源与环境学院,西藏林芝860000 [2]尼木县自然资源局,西藏尼木851300

出　　处：《种子》2024年第8期151-156,共6页Seed

基　　金：拉萨南北山造林主要乡土树种培育示范中心建设项目;西藏高原特色种养循环农业生态模式研究项目。

摘　　要：为筛选适合大花黄牡丹花粉活力检测的方法,以大花黄牡丹花粉为试验材料,采用离体培养法和染色法(TTC、碘-碘化钾、醋酸洋红)测定其花粉活力。结果表明,4种因素对大花黄牡丹花粉离体萌发率的影响依次为温度>蔗糖浓度>硼酸浓度>氯化钙浓度,其中温度和蔗糖浓度的主效应显著;现有因素水平下,1%琼脂+150 g/L蔗糖+20 mg/L氯化钙+50 mg/L硼酸+25℃恒温培养1.5 h是大花黄牡丹花粉离体培养的最佳条件。研究表明,花粉离体培养可快速、准确测定大花黄牡丹的花粉活力,碘-碘化钾染色法可用于大花黄牡丹花粉活力的快捷检测;TTC染色法和醋酸洋红染色法则不适用。In order to select a suitable method for the detection of Paeonia ludlowii pollen viability,using Paeonia ludlowii pollen as research object,the pollen viability was determined by culturing in vitro and staining methods(TTC,iodine-potassium iodide and carmine acetate).The results showed that the effect of four factors on pollen germinatin rate in vitro of Paeonia ludlowii from strong to weak was temperature,sucrose concentration,boric acid concentration,calcium chloride concentration,of which temperture and sucrose concentration had the most significant effect on pollen germination rate in vitro.Under the existing factors,1%AGAR+150 g/L sucrose+20 mg/L calcium chloride+50 mg/L boric acid at constant temperature of 25℃culturing for 1.5 hours was the best condition for in vitro culture of Paeonia ludlowii pollen.The comparison of four methods showed that in vitro pollen culture could be used for rapid and accurate determination of pollen viability,and iodine-potassium iodide staining could be used for rapid detection of pollen viability.TTC staining and acetic acid magenta staining rules were not applicable.

关 键 词：大花黄牡丹 花粉活力 离体培养 染色法 

分 类 号：S685.11[农业科学—观赏园艺]