以苯酚为电子供体驯化的反硝化污泥的脱氮特性  

Nitrogen removal characteristics of phenol-acclimated denitrifying sludge for denitrification with phenol as electron donor

作　　者：李默 张海雲 袁茹 张永明 LI Mo;ZHANG Haiyun;YUAN Ru;ZHANG Yongming(School of Environmental and Geographical Sciences,Shanghai Normal University,Shanghai 200234,China)

机构地区：[1]上海师范大学环境与地理科学学院,上海200234

出　　处：《上海师范大学学报（自然科学版中英文）》2024年第4期557-563,共7页Journal of Shanghai Normal University(Natural Sciences)

基　　金：国家自然科学基金(52300058)。

摘　　要：本研究以苯酚作为电子供体进行反硝化,驯化了一种能以苯酚为电子供体的反硝化污泥(PAS).当利用苯酚为电子供体进行反硝化时,其反硝化速率是传统葡萄糖驯化的反硝化污泥(GAS)的3.2倍.对2种反硝化污泥的高通量测序结果表明:PAS中微生物群落的Chao1指数要高于GAS,但GAS中微生物群落的Shannon指数要高于PAS.这表明PAS中微生物群落物种的丰富度高于GAS,但物种的多样性低于GAS.对2种反硝化污泥的微生物结构和相对丰度分析表明:PAS中含有的Denitratisoma丰度远高于GAS,这使PAS在利用苯酚作为电子供体进行反硝化时,明显优于GAS,因为Denitratisoma同时具有降解苯酚和反硝化的功能.In this work,a phenol-acclimated sludge(PAS)was obtained by acclimating activated sludge with phenol as electron donor.When phenol was used as electron donor for denitrification,the denitrification rate was as 3.2 folds as that with glucoseacclimated sludge(GAS).The high-throughput sequencing results for the two denitrifying sludges indicate that Chao1 index in PAS was higher than that in GAS,but Shannon index in GAS was higher than that in PAS.This suggests that species richness in PAS was higher than that in GAS,but species diversity in PAS was lower than that in GAS.The analysis for the microbial structure and relative abundance of two types of denitrifying sludge documented that the abundance of genus Denitratisoma in PAS is much higher than that in GAS,which let PAS be much superior to GAS in the denitrification with phenol as electron donor,because genus Denitratisoma had both the function of degrading phenol and denitrification.

关 键 词：反硝化 电子供体 苯酚 

分 类 号：X522[环境科学与工程—环境工程]