宏基因组二代测序诊断皮肤龟分枝杆菌感染1例  

Cutaneous Mycobacterium Chelonae Infection Diagnosed by Metagenomic Next-Generation Sequencing:A Case Report

作　　者：任露露 黄鹤群 范星[1,2] 肖风丽[1,2,3] REN Lulu;HUANG Hequn;FAN Xing;XIAO Fengli(Department of Dermatology and Venereology,the First Affiliated Hospital of Anhui Medical University,Hefei 230022,China;Key Laboratory of Dermatology,Ministry of Education of Anhui Medical University,Hefei 230022,China;Inflammation and Immune Mediated Diseases Laboratory of Anhui Province,Hefei 230022,China)

机构地区：[1]安徽医科大学第一附属医院皮肤性病科,安徽合肥230022 [2]安徽医科大学皮肤病研究所,皮肤病教育部重点实验室,安徽合肥230022 [3]炎症免疫性疾病安徽省实验室,安徽合肥230022

出　　处：《中国皮肤性病学杂志》2024年第9期1012-1017,共6页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金面上项目(82373481);安徽省高校自然科学基金重点项目(2023AH053302)。

摘　　要：目的探讨宏基因组二代测序(metagenomic next-generation sequencing,mNGS)在不明原因皮肤感染患者病原体检测中的作用。方法患者女,68岁,左膝红肿结节伴疼痛4个月,破溃20 d,提取患者病变皮肤组织样本进行宏基因组二代测序,对样本中微生物核酸序列进行分析,与病原数据库比对,鉴定病原微生物。结果mNGS检测到龟分枝杆菌(28个特异序列),并由PCR检测及Sanger测序验证,说明该皮肤组织感染的致病微生物为龟分枝杆菌。根据患者的临床症状,最终诊断为皮肤龟分枝杆菌感染。结论mNGS可辅助诊断少见病原微生物感染,为临床治疗提供方向。Objective To explore the role of metagenomic next-generation sequencing(mNGS)in pathogen detection of patients with skin infections of unknown origin.Methods A 68-year-old female patient presented with red,swollen nodules and pain in the left knee for 4 months,and ulceration for 20 days.mNGS was performed on the patient′s skin lesion samples,and the microbial nucleic acid sequences in the samples were analyzed and compared with the pathogen database to identify pathogenic microorganisms.Results mNGS detected Mycobacterium chelonae(28 specific sequences),which was verified by PCR detection and Sanger sequencing,indicating that the pathogenic microorganism causing the skin tissue infection was Mycobacterium chelonae.Based on the patient′s clinical symptoms,the final diagnosis was Mycobacterium chelonae skin infection.Conclusion mNGS can assist in the diagnosis of rare pathogenic microorganism infections and provide directions for clinical treatment.

关 键 词：龟分枝杆菌 非结核分枝杆菌 皮肤感染 宏基因组二代测序 

分 类 号：R754[医药卫生—皮肤病学与性病学]