Contrast-enhanced fluorescence microscope by LED integrated excitation cubes  

作　　者：Yuanhua Liu Xiang Zhang Fei Su Zhiyong Guo Dayong Jin 

机构地区：[1]UTS-SUSTech Joint Research Centre for Biomedical Materials and Devices,Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,Guangdong 518055,China [2]Institute for Biomedical Materials and Devices(IBMD),Faculty of Science,University of Technology Sydney,NSW 2007,Australia

出　　处：《Light(Advanced Manufacturing)》2023年第2期18-27,共10页光（先进制造）（英文）

基　　金：the Shenzhen Science and Technology Program(KQTD20170810110913065 and 20200925174735005).

摘　　要：Fluorescence microscopy is a powerful tool for scientists to observe the microscopic world,and the fluorescence excitation light source is one of the most critical components.To compensate for the short operation lifetime,integrated light sources,and low excitation efficiency of conventional light sources such as mercury,halogen,and xenon lamps,we designed an LED-integrated excitation cube(LEC)with a decentralized structure and high optical power density.Using a Fresnel lens,the light from the light-emitting diode(LED)was effectively focused within a 15 mm mounting distance to achieve high-efficiency illumination.LEC can be easily designed in the shape of fluorescence filter cubes for installation in commercial fluorescence microscopes.LECs’optical efficiency is 1–2 orders of magnitude higher than that of mercury lamps;therefore,high-quality fluorescence imaging with spectral coverage from UV to red can be achieved.By replacing conventional fluorescence filter cubes,LEC can be easily installed on any commercial fluorescence microscope.A built-in LEC driver can identify the types of LEDs in different spectral bands to adopt the optimal operating current and frequency of pulses.Moreover,high-contrast images can be achieved in pulse mode by time-gated imaging of long-lifetime luminescence.

关 键 词：LED-integrated excitation cube Filter cube Time-gated imaging Fluorescence microscopy 

分 类 号：TH742[机械工程—光学工程]