商桔梗多糖提取工艺优化及其吸湿保湿、抗氧化性能研究  

Study on Optimization of Extraction Process of Polysaccharide from Shang Platycodon and Its Moisture-Absorption-Retention and Antioxidant Properties

作　　者：陈卫 谷彩花 毕晶晶 叶兆伟[1,2] 张亚欣 白向茹 马晓辉 陈琼 CHEN Wei;GU Caihua;BI Jingjing;YE Zhaowei;ZHANG Yaxin;BAI Xiangru;MA Xiaohui;CHEN Qiong(School of Pharmacy,Xinyang Agriculture and Forestry University,Henan Xinyang 464000,China;Dabie Mountain Forest-Medicine Ecological Planting Engineering and Technology Research Center of Henan Province,Henan Xinyang 464000,China;School of Agronomy,Xinyang Agriculture and Forestry University,Henan Xinyang 464000,China)

机构地区：[1]信阳农林学院药学院,河南信阳464000 [2]河南省大别山林药生态种植工程技术研究中心,河南信阳464000 [3]信阳农林学院农学院,河南信阳464000

出　　处：《饲料工业》2024年第17期113-119,共7页Feed Industry

基　　金：河南省重大科技专项[201111310900];河南省重点研发专项[241111311400];河南省科技攻关项目[232102310364];河南省高等学校重点科研项目[22A150041];信阳农林学院青年基金项目[QN2022021]。

摘　　要：试验旨在优化商桔梗多糖的提取工艺,分析其吸湿保湿和抗氧化性能。采用正交试验优化超声波辅助水提醇沉法提取商桔梗多糖的提取工艺,并对提取的商桔梗多糖进行精制,用于吸湿保湿及抗氧化性能的测定分析。结果显示,在液料比20 mL/g、超声提取温度50℃、超声提取时间20 min时,多糖提取率达9.75%,为商桔梗多糖的最佳提取工艺。在相对湿度(RH)60%、81%情况下,吸湿率大小顺序均为丙三醇>海藻酸钠>商桔梗多糖;在干燥环境下,保湿率大小顺序为海藻酸钠>商桔梗多糖>丙三醇。商桔梗多糖能够对羟基自由基和DPPH自由基进行清除,且商桔梗多糖的浓度与清除能力呈正相关性。其中,在商桔梗多糖的浓度分别为2.0、10.0 mg/mL时,可以实现对羟基自由基和DPPH自由基的半数抑制。说明商桔梗多糖的吸湿性不强,但保湿性较好,且具有一定的抗氧化性能,可以开发为一种天然的动物饲料添加剂。The purpose of this study was to optimize the extraction process of polysaccharides from Shang Platycodon and to analyze its hygroscopic,moisturizing and antioxidant properties.Orthogonal experi⁃ments were used to optimize the extraction process of polysaccharides from Shang Platycodon by ultrasonic-assisted water extraction and alcohol precipitation.The polysaccharides extracted under the optimal extraction process were refined and used for the determination and analysis of hygroscopic moisturizing and antioxidant properties.The re⁃sults showed that the extraction rate of polysaccha⁃rides was 9.75%when the liquid-material ratio was 20 mL/g,the ultrasonic extraction tempera⁃ture was 50℃,and the ultrasonic extraction time was 20 min,which was the best extraction process for polysaccharides from Shang Platycodon.In the case of RH 60%and RH 81%,the order of mois⁃ture absorption rate was glycerol>sodium algi⁃nate>glycerol polysaccharides;and in dry envi⁃ronment,the moisture retention rate was sodium alginate>polysaccharides of bellflower>glycerol.Poly⁃saccharides from Shang Platycodon can scavenge hydroxyl radicals and DPPH free radicals,and the con⁃centration of polysaccharides is positively correlated with scavenging ability.Among them,when the con⁃centrations of polysaccharides were 2.0 mg/mL and 10.0 mg/mL,respectively,half of the hydroxyl radi⁃cals and DPPH radicals could be inhibited.Studies have shown that the polysaccharide from Shang Platycodon is not enough hygroscopic,but it has good moisturizing property and has certain antioxidant properties,which can be developed as a natural animal feed additive.

关 键 词：商桔梗多糖 超声波辅助 吸湿 保湿 抗氧化 饲料添加剂 

分 类 号：S816.7[农业科学—饲料科学]