宽谱散斑定制及其在单次曝光多色荧光超分辨显微鬼成像的应用  

Customization of Broadband Speckle and Its Application in Single-Shot Multi-Color Fluorescence Super-Resolution Microscopic Ghost Imaging

作　　者：陈丽 万霄汉 王鹏威 刘震涛[1,2,3,4] 吴建荣 韩申生[1,2,3,4,6] Chen Li;Wan Xiaohan;Wang Pengwei;Liu Zhentao;Wu Jianrong;Han Shensheng(Key Laboratory of Quantum Optics,Shanghai Institute of Optics and Fine Mechanics,Chinese Academy of Sciences,Shanghai201800,China;Center of Materials Science and Optoelectronics Engineering,University of Chinese Academy of Sciences,Beijing 100049,China;Wangzhijiang Innovation Center for Laser,Aerospace Laser Technology and System Department,Shanghai Institute of Optics and Fine Mechanics,Chinese Academy of Sciences,Shanghai 201800,China;Key Laboratory of Space Laser Communication and Detection Technology,Shanghai Institute of Optics and Fine Mechanics,Chinese Academy of Sciences,Shanghai 201800,China;Key Laboratory of Advanced Optical Manufacturing Technologies of Jiangsu Province,Soochow University,Suzhou 215006,Jiangsu,China;Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou 310024,Zhejiang,China)

机构地区：[1]中国科学院上海光学精密机械研究所量子光学重点实验室,上海201800 [2]中国科学院大学材料与光电研究中心,北京100049 [3]中国科学院上海光学精密机械研究所空天激光技术与系统部王之江创新中心,上海201800 [4]中国科学院上海光学精密机械研究所空间激光传输与探测技术重点实验室,上海201800 [5]苏州大学江苏省先进光学制造技术重点实验室,江苏苏州215006 [6]中国科学院大学杭州高等研究院,浙江杭州310024

出　　处：《光学学报》2024年第14期299-308,共10页Acta Optica Sinica

基　　金：江苏省先进光学制造技术重点实验室资助项目(KJS2337)。

摘　　要：针对现有的定制化散斑调制方法难以在特定宽谱波长范围、特定轴向距离范围内获得满足同一统计分布的散斑的不足,提出一种定制宽波段散斑调制的方法,并将其应用到单次曝光多色荧光超分辨显微鬼成像中。通过设计单次曝光多色荧光超分辨显微鬼成像系统的相位调制器在多个波长实现定制超瑞利散斑调制,仿真结果表明,所提方法不仅能显著提高多色荧光显微成像的时间和空间分辨率,还能够实现极低信噪比下的多色信号重构,有望应用到生物学中观察活细胞的动态过程。Objective In some practical applications, the general speckle that obeys the Rayleigh distribution cannot meet the application requirements. Therefore, it is necessary to customize speckles with a specific distribution. Recent studies on speckle customization are mainly generated by the illumination of the active laser light source. We explore a method for customizing speckles in a passive detection mode. However, it is difficult for existing customized speckle modulation methods to obtain the speckle with the same statistical distribution in a specific wide spectrum wavelength range and a specific axial distance range. Maintaining the speckle with the same statistical distribution in broadband is vital for multicolor imaging. To this end, we propose a method to customize broadband speckle modulation based on multi-wavelength inverse propagation theory and iterative algorithms.Methods We put forward a broadband speckle customization method for the passive detection mode. The multiwavelength inverse propagation theory based on Fresnel diffraction and iterative algorithms is adopted to optimize the phase of phase modulators. Firstly, the incident fields of all modulated wavelengths at the source plane propagate a distance to the modulator plane, and the modulated fields which are phases modulated by the phase modulator(randomly initialization) propagate a distance to the detection plane. Secondly, we update the amplitude of detected fields with the target modulation patterns(retaining its phase) and the updated fields of all modulated wavelengths propagate inversely to the modulator plane. Thirdly, the effect of initial incident fields is eliminated and the fields at the modulator plane over all wavelengths are averaged. Finally, the phase of modulators is updated and the iteration is performed until the modulation patterns of all wavelengths are target modulation patterns. The customized broadband speckles are generated at a certain axial distance by the illumination of the optimized phase modulator with an incohe

关 键 词：物理光学 定制散斑 宽波段 多色荧光显微 超瑞利散斑 低信噪比 

分 类 号：O436[机械工程—光学工程]