云南金沙江干热河谷土壤微生物群落结构特征及影响因素  

Soil microbial community structure and influencing factors in dry⁃hot valley region of Jinsha River,Yunnan Province

作　　者：刘顺 许格希[1,2] 陈健 陈淼[1,2] 史作民 周庆宏 蒋冬梅[4] LIU Shun;XU Gexi;CHEN Jian;CHEN Miao;SHI Zuomin;ZHOU Qinghong;JIANG Dongmei(Key Laboratory of Forest Ecology and Environment of National Forestry and Grassland Administration,Ecology and Nature Conservation Institute,Chinese Academy of Forestry,Beijing 100091,China;Sichuan Miyaluo Forest Ecosystem National Observation and Research Station,Lixian 623100,China;Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,China;Chuxiong Research Institute of Forestry and Grassland,Chuxiong 675005,China)

机构地区：[1]中国林业科学研究院森林生态环境与自然保护研究所,国家林业和草原局森林生态环境重点实验室,北京100091 [2]四川米亚罗森林生态系统国家定位观测研究站,理县623100 [3]南京林业大学南方现代林业协同创新中心,南京210037 [4]楚雄彝族自治州林业和草原科学研究所,楚雄675005

出　　处：《生态学报》2024年第16期7172-7182,共11页Acta Ecologica Sinica

基　　金：云南省科技人才与专家计划“史作民专家工作站”项目(202305AF150100);中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2021ZA002-2)。

摘　　要：金沙江干热河谷区是长江上游重要的生态屏障和典型生态脆弱区,对金沙江干热河谷土壤微生物群落结构变异特征及其驱动因素的了解仍不清楚。沿着金沙江选择5个地点的典型干热河谷植被,采用磷脂脂肪酸法研究了土壤微生物生物量和群落结构的空间变异,并探讨了气候、植被因子和土壤性质等对土壤微生物群落结构的影响。结果表明:不同研究点土壤总微生物生物量介于4.30—13.20 nmol/g,以细菌为主,其中革兰氏阳性菌(G+)占26.22%—33.65%,革兰氏阴性菌(G-)占18.35%—23.67%;真菌占9.02%—15.13%。不同研究点土壤微生物生物量、真菌/细菌和G+/G-差异显著,并且非度量多维尺度分析形成不同聚类,表明土壤微生物群落结构在研究点间发生了变化。方差分解结果揭示了土壤微生物群落结构的变异主要受土壤因子的驱动,其次是植被和气候因素。草本生物量、土壤硝态氮、全碳、pH和含水量对土壤微生物群落结构具有显著影响。气候因素中年均降雨量对微生物生物量呈负影响效应。揭示了不同研究点通过土壤性质驱动了土壤微生物群落结构的变异,并发现了草本生物量在其中的重要作用,对干热河谷区植被恢复具有重要的意义。Soil microbial communities play an important regulatory role in soil structural formation,nutrient cycling,and plant growth in terrestrial ecosystems,which are of great significance to the reestablishment of functions in degraded ecosystems.The dry⁃hot valley region of Jinsha River is an important ecological barrier in the upper reaches of the Yangtze River,and a typical ecologically fragile area.However,the understanding of variations in soil microbial community structure and driving factors in this region remains unclear.Here,the variations in soil microbial biomass and community structure of typical dry⁃hot valley across five sites along the Jinsha River basin were investigated using phospholipid fatty acid(PLFA)analysis.Further,the effects of climatic,vegetation and edaphic factors on soil microbial community structure were explored.The results showed that total soil microbial biomass ranged from 4.30 nmol/g to 13.20 nmol/g across the five sites.Bacterial biomass accounted for the largest proportion of total microbial biomass,with gram⁃positive bacterial(G+)and gram⁃negative bacterial(G-)biomass accounting for 26.22%—33.65%and 18.35%—23.67%,respectively.The proportion of fungal biomass in total microbial biomass was between 9.02%and 15.13%.These sites had significant difference in microbial biomass of total and specific functional group microorganisms,fungi/bacteria,G+/G-,cyclopropane fatty acids/monoenoic precursors,normal saturated fatty acids/monounsaturated fatty acids.Moreover,their soil microbial community structure formed different clusters based on the non⁃metric multidimensional scale(NMDS)analysis,suggesting significant differences among the sites.The results of variation partitioning analysis(VPA)revealed that the variation of soil microbial community structure was mainly driven by edaphic factors,followed by vegetation and climatic factors with the pure effects being 37%,16%and 2%,respectively.Specifically,herb biomass(P=0.002),soil NO_(3)^(-)(P=0.002),soil total carbon(P=0.003),pH(P

关 键 词：磷脂脂肪酸 河谷型萨王纳植被 草本生物量 金沙江 

分 类 号：S154.3[农业科学—土壤学]