野鸟源禽副黏病毒的流行病学调查及遗传进化分析  

Epidemiological investigation and genetic evolution analysis of avian paramyxoviruses derived from wild birds

作　　者：李乔乔 刑亚楠 胡斌 韩姝伊 高思潮 王继普 王业 何宏轩[2] 李文超[1] LI Qiaoqiao;XING Yanan;HU Bin;HAN Shuyi;GAO Sichao;WANG Jipu;WANG Ye;HE Hongxuan;LI Wenchao(College of Animal Science,Anhui Science and Technology University,Fengyang 233100,China;National Research Center for Wildlife-Borne Diseases,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100 [2]中国科学院动物研究所国家野生动物疫病研究中心,北京100101

出　　处：《安徽科技学院学报》2024年第5期8-14,共7页Journal of Anhui Science and Technology University

基　　金：国家科技基础资源调查专项(2022FY100903)。

摘　　要：目的:调查我国野生鸟类中禽副黏病毒(Avian Paramyxoviruses,APMV)的流行和进化趋势。方法:通过RT-PCR、鸡胚分毒、血凝试验、遗传进化分析对野鸟源样品进行APMV流行病学调查和进化分析。结果:1219份野鸟样品中共检测出9份APMV阳性样品,APMV感染率为0.74%。其中APMV-1有8份,感染率为0.66%;APMV-4有1份,感染率为0.08%。鸡胚分毒成功分离出1株鸽源PPMV-1,遗传进化分析显示其属于基因VI.2.1.1.2.2型,与近年来国内流行的基因型一致。结论:野鸟在APMV的传播中具有重要作用,应加强对野鸟源PPMV-1的监测,研究结果对可能因跨种传播导致的公共卫生事件进行早期预警防控提供理论依据。Objective:This study aimed to investigate the prevalence and evolution of APMV in wild birds in China.Methods:APMV was isolated from wild birds by RT-PCR,chicken embryo isolation,hemagglutination test and genetic evolution analysis.Results:A total of 9 APMV positive samples were detected in 1219 samples,and the infection rate was 0.74%.The positive rate of APMV-1 was 0.66%.One sample was positive for APMV-4,and the infection rate was 0.08%.A strain pigeon source PPMV-1 was successfully isolated from the chicken embryo toxicity.Phylogenetic analysis showed that the isolate obtained from the pigeon belonged to genotype VI.2.1.1.2.2,which was consistent with the genotype prevalent in China in recent years.Conclusion:Wild birds play an important role in the transmission of APMV,and monitoring of wild bird source PPMV-1 should be strengthened.The research results provided a theoretical basis for early warning and prevention of public health events that may be caused by cross species transmission.

关 键 词：APMV 分离鉴定 遗传进化分析 野鸟 

分 类 号：S855.3[农业科学—临床兽医学]