辣椒DNA快速提取方法优化  

Optimization of rapid extraction method of pepper DNA

作　　者：袁友志 施二荣 纪艺雪 汪玲娟 胡能兵 张雪平 叶梅荣 隋益虎 YUAN Youzhi;SHI Errong;JI Yixue;WANG Lingjuan;HU Nengbing;ZHANG Xueping;YE Meirong;SUI Yihu(College of Agriculture,Anhui Science and Technology University,Fengyang 233100,China)

机构地区：[1]安徽科技学院农学院,安徽凤阳233100

出　　处：《安徽科技学院学报》2024年第5期21-25,共5页Journal of Anhui Science and Technology University

基　　金：安徽省高校自然科学研究项目(2022AH051626);安徽省辣椒良种联合攻关项目;安徽省高校优秀青年人才支持计划重点项目(gxyqZD2017070);安徽科技学院稳定人才项目(NXWD201701);萧县禾盛种业有限公司横向项目(880499);安徽科技学院乡村振兴项目(811156)。

摘　　要：目的:获得一种能够快速提取辣椒基因组DNA的方法,为开展大量的辣椒分子育种工作奠定基础。方法:以CTAB法为对照,通过优化碱煮法的处理时间,不同组织DNA提取效果和保存时间,比较不同提取的DNA在SSR分子标记扩增结果。结果:与传统提取方法相比,NaOH和吐温-20处理3~5 min所提取的DNA扩增效果并无明显差异,4℃条件下可以保存1周左右,并且在植物根部也能获得较好的提取效果。结论:碱煮法所提取的辣椒DNA可以应用于分子标记辅助育种和杂交纯度鉴定等试验。该方法更加快速便捷。Objective:A method for rapid and high-throughput extraction of pepper genomic DNA was obtained,which laid a foundation for a large number of pepper molecular breeding work.Methods:By optimizing the treatment time of alkali boiling method,the DNA extraction effect and preservation time of different tissues of pepper were further studied,and the results of SSR molecular marker amplification were compared with those of DNA extracted by CTAB method.Results:Compared with the traditional extraction method,there was no significant difference in the amplification effect of DNA extracted by NaOH and Tween-20 treatment for 3-5 min,and it could be stored for about a week at 4℃.And it could also obtain better extraction effect in plant roots.Conclusion:The pepper DNA extracted by alkali boiling method could be applied to molecular marker-assisted breeding and hybrid purity identification.This method was more rapid and convenient.

关 键 词：碱煮法 辣椒 NAOH 快速提取 

分 类 号：S641.3[农业科学—蔬菜学]