丁香油包合物协同青霉素抗金黄色葡萄球菌  

Synergistic effect of clove oil inclusion complex with penicillin against Staphylococcus aureus

作　　者：史玉军 张孝林 SHI Yujun;ZHANG Xiaolin(College of Life and Health Science,Anhui Science and Technology University,Fengyang 233100,China)

机构地区：[1]安徽科技学院生命与健康科学学院,安徽凤阳233100

出　　处：《安徽科技学院学报》2024年第5期36-41,共6页Journal of Anhui Science and Technology University

基　　金：国家级大学生创新创业计划项目(202110879027);安徽省高校自然科学研究项目(2022AH040231)。

摘　　要：目的:研究丁香油包合物协同青霉素抗金黄色葡萄球菌的作用。方法:以包合率为指标,在考察包合温度、丁香油滴加速率和搅拌时间单因素基础上设计正交试验,确立1 g/mL羟丙基β-环糊精溶液中丁香油最佳包合工艺;以棋盘法测定丁香油包合物联合青霉素是否具有协同青霉素抗金黄色葡萄球菌作用。结果:羟丙基β-环糊精包被丁香油的最佳工艺为包合温度为60℃、丁香油滴加速度为0.04 mL/min、搅拌时间即包合时间为7.5 h。其最佳包合率达28.6%。棋盘法研究发现丁香油联合青霉素对金黄色葡萄球菌部分抑菌浓度指数(FIC)为0.375,小于协同作用FIC指数值0.5,因此,2种药具有协同抗金黄色葡萄球菌作用。结论:中药成分丁香油与青霉素联合使用可提高青霉素抗金黄色葡萄球菌活性。Objective:To investigate the synergistic effects of clove oil inclusion complex with penicillin against Staphylococcus aureus.Methods:The inclusion rate was used as an indicator to design an orthogonal test for establishing the optimal inclusion process of clove oil in 1 g/mL hydroxypropylβ-cyclodextrin solution.A chessboard method was used to determine whether clove oil inclusion complex combined with penicillin could enhance the anti-Staphylococcus aureus activity of the two drugs.Results:The optimal process for coating clove oil with hydroxypropylβ-cyclodextrin was as follows:the inclusion temperature was 60℃,and the droplet rate of clove oil was 0.04 mL/min,the mixing inclusion time was 7.5 h.The optimal inclusion rate was 28.6%.The test of checkerboard study found that the partial inhibitory concentration(FIC)index of clove oil combined with penicillin against Staphylococcus aureus was 0.375,which was lower than the FIC index of synergistic effect of 0.5.Therefore,the two drugs had synergistic effects of anti-Staphylococcus aureus.Conclusion:The clove oil,a traditional Chinese medicine ingredient,cormbined with penicillin could enhance the anti Staphylococcus aureus activity of penicillin.

关 键 词：丁香油 羟丙基β-环糊精 包合物 青霉素 协同抗菌作用 金黄色葡萄球菌 

分 类 号：R931.71[医药卫生—生药学]