A Two-color Single-molecule Sequencing Platform and Its Clinical Applications  

作　　者：Fang Chen Bin Liu Meirong Chen Zefei Jiang Zhiliang Zhou Ping Wu Meng Zhang Huan Jin Linsen Li Liuyan Lu Huan Shang Lei Liu Weiyue Chen Jianfeng Xu Ruitao Sun Guangming Wang Jiao Zheng Jifang Qi Bo Yang Lidong Zeng Yan Li Hui Lv Nannan Zhao Wen Wang Jinsen Cai Yongfeng Liu Weiwei Luo Juan Zhang Yanhua Zhang Jicai Fan Haitao Dan Xuesen He Wei Huang Lei Sun Qin Yan 

机构地区：[1]GeneMind Biosciences Co.,Ltd.,Shenzhen 518000,China

出　　处：《Genomics, Proteomics & Bioinformatics》2024年第1期169-175,共7页基因组蛋白质组与生物信息学报（英文版）

摘　　要：DNA sequencers have become increasingly important research and diagnostic tools over the past 20 years.In this study,we developed a single-molecule desktop sequencer,GenoCare 1600(GenoCare),which utilizes amplification-free library preparation and two-color sequencing-by-synthesis chemistry,making it more user-friendly compared with previous single-molecule sequencing platforms for clinical use.Using the GenoCare platform,we sequenced an Escherichia coli standard sample and achieved a consensus accuracy exceeding 99.99%.We also evaluated the sequencing performance of this platform in microbial mixtures and coronavirus disease 2019(COVID-19)samples from throat swabs.Our findings indicate that the GenoCare platform allows for microbial quantitation,sensitive identification of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)virus,and accurate detection of virus mutations,as confirmed by Sanger sequencing,demonstrating its remarkable potential in clinical application.

关 键 词：GenoCare Single-molecule sequencing SARS-CoV-2 Microbial quantitation Variant detection 

分 类 号：Q7[生物学—分子生物学] Q81